BMRB Entry 19902
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19902
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Title: Solution NMR structure of the reovirus p15 fusion-associated small transmembrane (FAST) protein fusion-inducing lipid packing sensor (FLiPS) motif in dodecyl phosphocholine (DPC) micelles
Deposition date: 2014-04-09 Original release date: 2014-04-22
Authors: Sarker, Muzaddid; Duncan, Roy; Read, Jolene; Rainey, Jan
Citation: Read, Jolene; Clancy, Eileen; Sarker, Muzaddid; Langelaan, David; Rainey, Jan; Duncan, Roy. "Novel helix-loop-helix fusion-inducing lipid packing sensor (FLiPS) for cell-cell fusion" Not known ., .-..
Assembly members:
entity_1, polymer, 21 residues, 1941.335 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XLGLLSYGAGVASLPLLNVI
A
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 39 |
| 15N chemical shifts | 20 |
| 1H chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 21 residues - 1941.335 Da.
| 1 | ACE | LEU | GLY | LEU | LEU | SER | TYR | GLY | ALA | GLY | ||||
| 2 | VAL | ALA | SER | LEU | PRO | LEU | LEU | ASN | VAL | ILE | ||||
| 3 | ALA |
Samples:
sample_1: entity_1 1 mM; DPC 150 mM; DSS 0.5 mM; sodium azide 0.2 mM; sodium acetate, [U-2H], 20 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - refinement
TOPSPIN v3.1, Bruker Biospin - processing
SPARKY v3.110, Goddard - chemical shift assignment
ProcheckNMR v3.5.4, Laskowski and MacArthur - validation
UCSF_CHIMERA v1.8, NIH - structure viewing
Molmol v2K.2, Koradi, Billeter and Wuthrich - ensemble viewing
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts