BMRB Entry 19952
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19952
MolProbity Validation Chart
NMR-STAR file interactive viewer.
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Title: Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)
Deposition date: 2014-05-05 Original release date: 2014-05-20
Authors: Letourneau, Danny; Bedard, Mikael; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre
Citation: Letourneau, Danny; Bedard, Mikael; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre. "Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)" Not known ., .-..
Assembly members:
StAR_D6, polymer, 220 residues, 25037.572 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
StAR_D6: MDFKAIAQQTAQEVLGYNRD
TSGWKVVKTSKKITVSSKAS
RKFHGNLYRVEGIIPESPAK
LSDFLYQTGDRITWDKSLQV
YNMVHRIDSDTFICHTITQS
FAVGSISPRDFIDLVYIKRY
EGNMNIISSKSVDFPEYPPS
SNYIRGYNHPCGFVCSPMEE
NPAYSKLVMFVQTEMRGKLS
PSIIEKTMPSNLVNFILNAK
DGIKAHRTPSRRGFHHNSHS
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 878 |
| 15N chemical shifts | 201 |
| 1H chemical shifts | 1355 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | StAR D6 | 1 |
Entities:
Entity 1, StAR D6 220 residues - 25037.572 Da.
| 1 | MET | ASP | PHE | LYS | ALA | ILE | ALA | GLN | GLN | THR | |
| 2 | ALA | GLN | GLU | VAL | LEU | GLY | TYR | ASN | ARG | ASP | |
| 3 | THR | SER | GLY | TRP | LYS | VAL | VAL | LYS | THR | SER | |
| 4 | LYS | LYS | ILE | THR | VAL | SER | SER | LYS | ALA | SER | |
| 5 | ARG | LYS | PHE | HIS | GLY | ASN | LEU | TYR | ARG | VAL | |
| 6 | GLU | GLY | ILE | ILE | PRO | GLU | SER | PRO | ALA | LYS | |
| 7 | LEU | SER | ASP | PHE | LEU | TYR | GLN | THR | GLY | ASP | |
| 8 | ARG | ILE | THR | TRP | ASP | LYS | SER | LEU | GLN | VAL | |
| 9 | TYR | ASN | MET | VAL | HIS | ARG | ILE | ASP | SER | ASP | |
| 10 | THR | PHE | ILE | CYS | HIS | THR | ILE | THR | GLN | SER | |
| 11 | PHE | ALA | VAL | GLY | SER | ILE | SER | PRO | ARG | ASP | |
| 12 | PHE | ILE | ASP | LEU | VAL | TYR | ILE | LYS | ARG | TYR | |
| 13 | GLU | GLY | ASN | MET | ASN | ILE | ILE | SER | SER | LYS | |
| 14 | SER | VAL | ASP | PHE | PRO | GLU | TYR | PRO | PRO | SER | |
| 15 | SER | ASN | TYR | ILE | ARG | GLY | TYR | ASN | HIS | PRO | |
| 16 | CYS | GLY | PHE | VAL | CYS | SER | PRO | MET | GLU | GLU | |
| 17 | ASN | PRO | ALA | TYR | SER | LYS | LEU | VAL | MET | PHE | |
| 18 | VAL | GLN | THR | GLU | MET | ARG | GLY | LYS | LEU | SER | |
| 19 | PRO | SER | ILE | ILE | GLU | LYS | THR | MET | PRO | SER | |
| 20 | ASN | LEU | VAL | ASN | PHE | ILE | LEU | ASN | ALA | LYS | |
| 21 | ASP | GLY | ILE | LYS | ALA | HIS | ARG | THR | PRO | SER | |
| 22 | ARG | ARG | GLY | PHE | HIS | HIS | ASN | SER | HIS | SER |
Samples:
STARD6: StAR_D6, [U-100% 13C; U-100% 15N], 1 mM
CondSet1: ionic strength: 0.100 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.150 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | STARD6 | isotropic | CondSet1 |
| 3D C(CO)NH | STARD6 | isotropic | CondSet1 |
| 3D HNCO | STARD6 | isotropic | CondSet1 |
| 2D 1H-15N HSQC | STARD6 | isotropic | CondSet1 |
| 3D HN(CO)CA | STARD6 | isotropic | CondSet1 |
| 3D HNHA | STARD6 | isotropic | CondSet1 |
| 3D H(CCO)NH | STARD6 | isotropic | CondSet1 |
| 3D 1H-15N NOESY | STARD6 | isotropic | CondSet1 |
| 3D HCCH-TOCSY | STARD6 | isotropic | CondSet1 |
| 2D 1H-13C HSQC | STARD6 | isotropic | CondSet1 |
| 3D 1H-13C NOESY aliphatic | STARD6 | isotropic | CondSet1 |
| 2D 1H-13C HSQC aromatic | STARD6 | isotropic | CondSet1 |
| 3D 1H-13C NOESY aromatic | STARD6 | isotropic | CondSet1 |
| 2D 1H-13C HSQC aliphatic | STARD6 | isotropic | CondSet1 |
Software:
CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - Structure calculation, refinement
CcpNmr_Analysis v2.2.2, CCPN - Assignment
DANGLE v1.1, CCPN - Dihedral Restraints from Chemical Shifts
NMR_Pipe v2.0, NMR Pipe - file conversion
ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - Structure calculation, refinement
NMR spectrometers:
- Varian UnityInova 600 MHz
Related Database Links:
| UniProt | P59095 |
| BMRB | 18777 |
| PDB | |
| DBJ | BAI46405 |
| GB | AAI40309 AAI46469 AAL89655 EAW63002 EAW63004 |
| REF | NP_631910 XP_011524122 XP_011524123 XP_014197853 XP_523930 |
| SP | P59095 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts