BMRB Entry 19957
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19957
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Title: Assignment of DNA-MC1 protein complex PubMed: 25212183
Deposition date: 2014-05-07 Original release date: 2014-10-14
Authors: Loth, Karine; Landon, Celine; Paquet, Francoise
Citation: Loth, Karine; Landon, Celine; Paquet, Francoise. "Chemical shifts assignments of the archaeal MC1 protein and a strongly bent 15 base pairs DNA duplex in complex" Biomol. NMR Assignments ., .-. (2014).
Assembly members:
MC1, polymer, 93 residues, Formula weight is not available
DNA1, polymer, 15 residues, Formula weight is not available
DNA2, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: E. Coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MC1: SNTRNFVLRDEDGNEHGVFT
GKQPRQAALKAANRGSGTKA
NPDIIRLRERGTKKVHVFKA
WKEIVDAPKNRPAWMPEKIS
KPFVKKERIEKLE
DNA1: AAAAACACACACCCA
DNA2: TGGGTGTGTGTTTTT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 78 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 873 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MC1 | 1 |
| 2 | DNA1 | 2 |
| 3 | DNA2 | 3 |
Entities:
Entity 1, MC1 93 residues - Formula weight is not available
| 1 | SER | ASN | THR | ARG | ASN | PHE | VAL | LEU | ARG | ASP | ||||
| 2 | GLU | ASP | GLY | ASN | GLU | HIS | GLY | VAL | PHE | THR | ||||
| 3 | GLY | LYS | GLN | PRO | ARG | GLN | ALA | ALA | LEU | LYS | ||||
| 4 | ALA | ALA | ASN | ARG | GLY | SER | GLY | THR | LYS | ALA | ||||
| 5 | ASN | PRO | ASP | ILE | ILE | ARG | LEU | ARG | GLU | ARG | ||||
| 6 | GLY | THR | LYS | LYS | VAL | HIS | VAL | PHE | LYS | ALA | ||||
| 7 | TRP | LYS | GLU | ILE | VAL | ASP | ALA | PRO | LYS | ASN | ||||
| 8 | ARG | PRO | ALA | TRP | MET | PRO | GLU | LYS | ILE | SER | ||||
| 9 | LYS | PRO | PHE | VAL | LYS | LYS | GLU | ARG | ILE | GLU | ||||
| 10 | LYS | LEU | GLU |
Entity 2, DNA1 15 residues - Formula weight is not available
| 1 | DA | DA | DA | DA | DA | DC | DA | DC | DA | DC | ||||
| 2 | DA | DC | DC | DC | DA |
Entity 3, DNA2 15 residues - Formula weight is not available
| 1 | DT | DG | DG | DG | DT | DG | DT | DG | DT | DG | ||||
| 2 | DT | DT | DT | DT | DT |
Samples:
sample_1: MC1, [U-15N], 1.18 mM; DNA1, [U-13C; U-15N], 1.18 mM; DNA2 1.18 mM; sodium phosphate 10 mM; sodium chloride 100 mM; EDTA 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 299 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance III HD 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts