BMRB Entry 19960
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19960
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Title: Solution structure of the human chemokine CCL19
Deposition date: 2014-05-09 Original release date: 2015-06-08
Authors: Veldkamp, C.; Peterson, F.; Gabel-Eissens, S.; Gillitzer, M.
Citation: Veldkamp, C.; Peterson, F.; Gabel-Eissens, S.; Gillitzer, M.. "Solution structure of the human chemokine CCL19" To be published ., .-..
Assembly members:
Human_Chemokine_CCL19, polymer, 77 residues, 8814.347 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Human_Chemokine_CCL19: GTNDAEDCCLSVTQKPIPGY
IVRNFHYLLIKDGCRVPAVV
FTTLRGRQLCAPPDQPWVER
IIQRLQRTSAKMKRRSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 267 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 557 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human Chemokine CCL19 | 1 |
Entities:
Entity 1, Human Chemokine CCL19 77 residues - 8814.347 Da.
| 1 | GLY | THR | ASN | ASP | ALA | GLU | ASP | CYS | CYS | LEU | ||||
| 2 | SER | VAL | THR | GLN | LYS | PRO | ILE | PRO | GLY | TYR | ||||
| 3 | ILE | VAL | ARG | ASN | PHE | HIS | TYR | LEU | LEU | ILE | ||||
| 4 | LYS | ASP | GLY | CYS | ARG | VAL | PRO | ALA | VAL | VAL | ||||
| 5 | PHE | THR | THR | LEU | ARG | GLY | ARG | GLN | LEU | CYS | ||||
| 6 | ALA | PRO | PRO | ASP | GLN | PRO | TRP | VAL | GLU | ARG | ||||
| 7 | ILE | ILE | GLN | ARG | LEU | GLN | ARG | THR | SER | ALA | ||||
| 8 | LYS | MET | LYS | ARG | ARG | SER | SER |
Samples:
sample: Human Chemokine CCL19, [U-100% 13C; U-100% 15N], 0.515 mM; D2O, [U-99% 2H], 10%; dueterated MES, [U-100% 2H], 25 mM
sample_conditions_1: ionic strength: 6 mM; pH: 5.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample | isotropic | sample_conditions_1 |
| 3D 13C-separated NOESY | sample | isotropic | sample_conditions_1 |
| 3D 13C-separated NOESY (AROMATIC) | sample | isotropic | sample_conditions_1 |
Software:
Xplor-NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement
TopSpin v3.1, Bruker - collection
nmrPipe v2007, Delagio,F. et al. - processing
XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis
GARANT v2.1, C. Bartels - data analysis
CARA v1.8.4, Keller, R. - data analysis
Cyana v3.1, Guntert, P. - structural calculation
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA20383 BAG34886 BAJ20579 |
| EMBL | CAA11307 CAG33149 |
| GB | AAC23905 AAC50944 AAH27968 AAS20190 ABK41949 |
| REF | NP_006265 XP_001164037 XP_002819711 XP_003829861 XP_004048020 |
| SP | Q99731 |
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