BMRB Entry 25076
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25076
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Title: Solid-state NMR resonance assignments of the filament-forming CARD domain of the innate immunity signaling protein MAVS
Deposition date: 2014-07-04 Original release date: 2015-08-31
Authors: He, Lichun; Luehrs, Thorsten; Ritter, Christiane
Citation: He, Lichun; Luehrs, Thorsten; Ritter, Christiane. "Solid-state NMR resonance assignments of the filament-forming CARD domain of the innate immunity signaling protein MAVS" Biomol. NMR Assignments ., .-..
Assembly members:
MAVS_CARD, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MAVS_CARD: GSMPFAEDKTYKYICRNFSN
FCNVDVVEILPYLPCLTARD
QDRLRATCTLSGNRDTLWHL
FNTLQRRPGWVEYFIAALRG
CELVDLADEVASVYQSYQPR
TS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 466 |
| 15N chemical shifts | 105 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MAVS CARD | 1 |
Entities:
Entity 1, MAVS CARD 102 residues - Formula weight is not available
| 1 | GLY | SER | MET | PRO | PHE | ALA | GLU | ASP | LYS | THR | ||||
| 2 | TYR | LYS | TYR | ILE | CYS | ARG | ASN | PHE | SER | ASN | ||||
| 3 | PHE | CYS | ASN | VAL | ASP | VAL | VAL | GLU | ILE | LEU | ||||
| 4 | PRO | TYR | LEU | PRO | CYS | LEU | THR | ALA | ARG | ASP | ||||
| 5 | GLN | ASP | ARG | LEU | ARG | ALA | THR | CYS | THR | LEU | ||||
| 6 | SER | GLY | ASN | ARG | ASP | THR | LEU | TRP | HIS | LEU | ||||
| 7 | PHE | ASN | THR | LEU | GLN | ARG | ARG | PRO | GLY | TRP | ||||
| 8 | VAL | GLU | TYR | PHE | ILE | ALA | ALA | LEU | ARG | GLY | ||||
| 9 | CYS | GLU | LEU | VAL | ASP | LEU | ALA | ASP | GLU | VAL | ||||
| 10 | ALA | SER | VAL | TYR | GLN | SER | TYR | GLN | PRO | ARG | ||||
| 11 | THR | SER |
Samples:
sample_1: MAVS CARD, [U-99% 13C; U-99% 15N], 20 mM; H2O, Natural Abundance, 100%
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DARR | sample_1 | solid | sample_conditions_1 |
| 3D NCACX | sample_1 | solid | sample_conditions_1 |
| 3D NCOCX | sample_1 | solid | sample_conditions_1 |
| 3D CANCO | sample_1 | solid | sample_conditions_1 |
Software:
CcpNmr, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 25109 |
| PDB | |
| DBJ | BAA86585 BAB14684 BAC77356 BAC85473 BAE79738 |
| GB | AAH44952 AAZ80417 ABA19229 ABA54890 ABR24162 |
| REF | NP_065797 XP_003821366 XP_008973040 XP_008973041 XP_009435017 |
| SP | Q7Z434 |