BMRB Entry 25147
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25147
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Title: Struture of -24 DNA binding domain of sigma 54 from E. coli
Deposition date: 2014-08-12 Original release date: 2015-08-24
Authors: Wemmer, David; Gao, Zhijuan; Pelton, Jeffrey
Citation: Gao, Zhijuan; Pelton, Jeffrey; Wemmer, David. "Struture of -24 DNA binding domain of sigma 54 from E. coli" Not known ., .-..
Assembly members:
entity, polymer, 84 residues, 9160.573 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: EASSTAIRALVKKLIAAENP
AKPLSDSKLTSLLSEQGIMV
ARRTVAKYRESLSIPPSNQR
KQLVANSSSVDKLAAALEHH
HHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 281 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 502 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 84 residues - 9160.573 Da.
| 1 | GLU | ALA | SER | SER | THR | ALA | ILE | ARG | ALA | LEU | ||||
| 2 | VAL | LYS | LYS | LEU | ILE | ALA | ALA | GLU | ASN | PRO | ||||
| 3 | ALA | LYS | PRO | LEU | SER | ASP | SER | LYS | LEU | THR | ||||
| 4 | SER | LEU | LEU | SER | GLU | GLN | GLY | ILE | MET | VAL | ||||
| 5 | ALA | ARG | ARG | THR | VAL | ALA | LYS | TYR | ARG | GLU | ||||
| 6 | SER | LEU | SER | ILE | PRO | PRO | SER | ASN | GLN | ARG | ||||
| 7 | LYS | GLN | LEU | VAL | ALA | ASN | SER | SER | SER | VAL | ||||
| 8 | ASP | LYS | LEU | ALA | ALA | ALA | LEU | GLU | HIS | HIS | ||||
| 9 | HIS | HIS | HIS | HIS |
Samples:
sample_1: entity mM; sodium phosphate 20 mM; sodium chloride 200 mM; DTT 1 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAA02315 BAB37504 BAE73474 BAE77246 BAG79010 |
| EMBL | CAA81617 CAD07837 CAP77662 CAQ33535 CAR00164 |
| GB | AAA27224 AAA58004 AAA62790 AAB60163 AAC76234 |
| PIR | AG0905 |
| REF | NP_312108 NP_417669 NP_457699 NP_462230 NP_709001 |
| SP | P24255 P26979 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts