BMRB Entry 25148
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25148
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Title: NTD-NUSA
Deposition date: 2014-08-12 Original release date: 2015-02-16
Authors: Mobli, Mehdi; Lewis, Peter
Citation: Mobli, Mehdi; Ma, Cong; Yang, Xiao; Keller, Andrew; King, Glenn; Lewis, Peter. "The Mechanism Of Transcription Pausing Enhancement By NusA" Not known ., .-..
Assembly members:
NTD-NUSA, polymer, 124 residues, 14019.991 Da.
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NTD-NUSA: MSSELLDALTILEKEKGISK
EIIIEAIEAALISAYKRNFN
QAQNVRVDLNRETGSIRVFA
RKDVVDEVYDQRLEISIEEA
QGIHPEYMVGDVVEIEVTPK
DFGRIAAQTAKQVVTQRVRE
AERG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 878 |
| 13C chemical shifts | 530 |
| 15N chemical shifts | 121 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NTD-NUSA | 1 |
Entities:
Entity 1, NTD-NUSA 124 residues - 14019.991 Da.
| 1 | MET | SER | SER | GLU | LEU | LEU | ASP | ALA | LEU | THR | ||||
| 2 | ILE | LEU | GLU | LYS | GLU | LYS | GLY | ILE | SER | LYS | ||||
| 3 | GLU | ILE | ILE | ILE | GLU | ALA | ILE | GLU | ALA | ALA | ||||
| 4 | LEU | ILE | SER | ALA | TYR | LYS | ARG | ASN | PHE | ASN | ||||
| 5 | GLN | ALA | GLN | ASN | VAL | ARG | VAL | ASP | LEU | ASN | ||||
| 6 | ARG | GLU | THR | GLY | SER | ILE | ARG | VAL | PHE | ALA | ||||
| 7 | ARG | LYS | ASP | VAL | VAL | ASP | GLU | VAL | TYR | ASP | ||||
| 8 | GLN | ARG | LEU | GLU | ILE | SER | ILE | GLU | GLU | ALA | ||||
| 9 | GLN | GLY | ILE | HIS | PRO | GLU | TYR | MET | VAL | GLY | ||||
| 10 | ASP | VAL | VAL | GLU | ILE | GLU | VAL | THR | PRO | LYS | ||||
| 11 | ASP | PHE | GLY | ARG | ILE | ALA | ALA | GLN | THR | ALA | ||||
| 12 | LYS | GLN | VAL | VAL | THR | GLN | ARG | VAL | ARG | GLU | ||||
| 13 | ALA | GLU | ARG | GLY |
Samples:
sample_1: NTD-NUSA, [U-99% 13C; U-99% 15N], 400 uM; HEPES 10 mM; sodium chloride 150 mM
sample_conditions_1: temperature: 298 K; pH: 7.8; pressure: 1 atm; ionic strength: 0.16 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
TOPSPIN v3.1, Bruker Biospin - collection
Rowland_NMR_Toolkit v3, J. C. Hoch & A. S. Stern - processing
TALOS v+, Cornilescu, Delaglio and Bax - data analysis
CCPNMR v2.2, CCPN - peak picking
NMR spectrometers:
- Bruker Avance 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAI85290 BAM52307 BAM57883 GAK80641 |
| EMBL | CAA79231 CAB13533 CCU58233 CEI56842 CEJ77249 |
| GB | ADM37756 ADV96687 AEP86650 AEP90813 AFI28344 |
| REF | NP_389542 WP_003220941 WP_003231912 WP_010328008 WP_010334282 |
| SP | P32727 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts