BMRB Entry 25181
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25181
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Title: Three-Dimensional Structure and Interaction Studies of Hepatitis C Virus p7 in 1,2-Dihexanoyl-sn-glycero-3-phosphocholine by Solution Nuclear Magnetic Resonance PubMed: 23841474
Deposition date: 2014-08-29 Original release date: 2014-10-07
Authors: Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Opella, Stanley
Citation: Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Opella, Stanley. "Three-dimensional structure and interaction studies of hepatitis C virus p7 in 1,2-dihexanoyl-sn-glycero-3-phosphocholine by solution nuclear magnetic resonance." Biochemistry 52, 5295-5303 (2013).
Assembly members:
entity, polymer, 63 residues, 6693.997 Da.
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 420174 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: ALENLVVLNAASVAGAHGIL
SFLVFFSAAWYIKGRLAPGA
AYAFYGVWPLLLLLLALPPR
AYA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 61 |
| 1H chemical shifts | 58 |
| 15N chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 63 residues - 6693.997 Da.
| 1 | ALA | LEU | GLU | ASN | LEU | VAL | VAL | LEU | ASN | ALA | ||||
| 2 | ALA | SER | VAL | ALA | GLY | ALA | HIS | GLY | ILE | LEU | ||||
| 3 | SER | PHE | LEU | VAL | PHE | PHE | SER | ALA | ALA | TRP | ||||
| 4 | TYR | ILE | LYS | GLY | ARG | LEU | ALA | PRO | GLY | ALA | ||||
| 5 | ALA | TYR | ALA | PHE | TYR | GLY | VAL | TRP | PRO | LEU | ||||
| 6 | LEU | LEU | LEU | LEU | LEU | ALA | LEU | PRO | PRO | ARG | ||||
| 7 | ALA | TYR | ALA |
Samples:
sample_1: p7, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; DHPC 125 mM; D2O 10%
sample_2: p7, [U-100% 15N], 0.5 mM; DHPC 125 mM; D2O 10%; H2O 90%; polyacrylamide gel 6%
sample_conditions_1: temperature: 323 K; pH: 4.0; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N HSQC NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 18863 25178 |
| PDB | |
| DBJ | BAA01583 BAA02756 |
| EMBL | CAA43792 |
| GB | AAC15722 AAC15723 AAC15724 AAC15725 AAC15726 |
| SP | O92972 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts