BMRB Entry 25203
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25203
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Title: Changing ABRA protein peptide to fit the HLA-DR B1*0301 molecule renders it protection-inducing PubMed: 15313182
Deposition date: 2014-09-04 Original release date: 2014-10-07
Authors: Salazar, Luz M.; Alba, Martha P.; Curtidor, Hernando; Bermudez, Adriana; Vargas, Luis E.; Rivera, Zuly J.; Patarroyo, Manuel E.
Citation: Salazar, Luz M.; Alba, Martha P.; Curtidor, Hernando; Bermudez, Adriana; Vargas, Luis E.; Rivera, Zuly J.; Patarroyo, Manuel E.. "Changing ABRA protein peptide to fit into HLA-DRB1*0301 molecule renders it protection-inducing" Biochem. Biophys. Res. Commun. 322, 119-125 (2014).
Assembly members:
entity, polymer, 20 residues, 2502.966 Da.
Natural source: Common Name: Malaria parasite Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: KMNMLKENVDYIQKNQNLFK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 20 residues - 2502.966 Da.
| 1 | LYS | MET | ASN | MET | LEU | LYS | GLU | ASN | VAL | ASP | |
| 2 | TYR | ILE | GLN | LYS | ASN | GLN | ASN | LEU | PHE | LYS |
Samples:
sample_1: entity7 – 10 mM; water 70%; trifluoroethanol 30%
sample_conditions_1: temperature: 295 K; pH: 3.7; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
InsightII, Accelrys Software Inc. - geometry optimization, refinement
NMR spectrometers:
- Bruker DRX 600 MHz