BMRB Entry 25214
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25214
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Title: HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1
Deposition date: 2014-09-10 Original release date: 2014-12-15
Authors: Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.
Citation: Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.. "HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1" Not known ., .-..
Assembly members:
PROTEIN_THO1, polymer, 67 residues, 7490.4826 Da.
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PROTEIN_THO1: GSALSPEEIKAKALDLLNKK
LHRANKFGQDQADIDSLQRQ
INRVEKFGVDLNSKLAEELG
LVSRKNE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 496 |
| 13C chemical shifts | 225 |
| 15N chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PROTEIN THO1 | 1 |
Entities:
Entity 1, PROTEIN THO1 67 residues - 7490.4826 Da.
| 1 | GLY | SER | ALA | LEU | SER | PRO | GLU | GLU | ILE | LYS | ||||
| 2 | ALA | LYS | ALA | LEU | ASP | LEU | LEU | ASN | LYS | LYS | ||||
| 3 | LEU | HIS | ARG | ALA | ASN | LYS | PHE | GLY | GLN | ASP | ||||
| 4 | GLN | ALA | ASP | ILE | ASP | SER | LEU | GLN | ARG | GLN | ||||
| 5 | ILE | ASN | ARG | VAL | GLU | LYS | PHE | GLY | VAL | ASP | ||||
| 6 | LEU | ASN | SER | LYS | LEU | ALA | GLU | GLU | LEU | GLY | ||||
| 7 | LEU | VAL | SER | ARG | LYS | ASN | GLU |
Samples:
sample_1: PROTEIN THO1, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 120.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | solution | sample_conditions_1 |
| 1H-13C HSQC | sample_1 | solution | sample_conditions_1 |
| HNCACB | sample_1 | solution | sample_conditions_1 |
| CBCA(CO)NH | sample_1 | solution | sample_conditions_1 |
| HNCO | sample_1 | solution | sample_conditions_1 |
| HN(CA)CO | sample_1 | solution | sample_conditions_1 |
| HNHB | sample_1 | solution | sample_conditions_1 |
| 1H-NOESY | sample_1 | solution | sample_conditions_1 |
| 1H-DQF-COSY | sample_1 | solution | sample_conditions_1 |
| 1H-TOCSY | sample_1 | solution | sample_conditions_1 |
Software:
Ansig vany, Kraulis - chemical shift assignment
AutoDep v4.3, PDBe - chemical shift assignment
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, refinement
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| UNP | THO1_YEAST |
| PDB | |
| DBJ | GAA22895 |
| EMBL | CAY79235 |
| GB | AAB64599 AAS56105 AHY75622 AJU40024 AJU40279 |
| REF | NP_010985 |
| SP | P40040 |
| TPG | DAA07722 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts