BMRB Entry 25217
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25217
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Title: 1H, 13C, and 15N Chemical Shift Assignments for AUX/IAA17 PubMed: 25512488
Deposition date: 2014-09-11 Original release date: 2014-12-15
Authors: Suh, Jeong-Yong; Han, Mookyoung
Citation: Han, Mookyoung; Park, Yangshin; Kim, Iktae; Kim, Eun-Hee; Yu, Tae-Kyung; Rhee, Sangkee; Suh, Jeong-Yong. "Structural basis for the auxin-induced transcriptional regulation by Aux/IAA17" Proc. Natl. Acad. Sci. U. S. A. 111, 18613-18618 (2014).
Assembly members:
Aux-IAA17, polymer, 113 residues, 12801.768 Da.
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Aux-IAA17: GGPEAAAFVKVSMDGAPYLR
KIDLRMYKSYDELSNALSNM
FSSFTMGKHGGEEGMIDFMN
ERKLMDLVNSWDYVPSYENK
DGNWMLVGDVPWPMFVDTCK
RLRLMKGSDAIGL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 602 |
| 13C chemical shifts | 428 |
| 15N chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AUX IAA17 | 1 |
Entities:
Entity 1, AUX IAA17 113 residues - 12801.768 Da.
| 1 | GLY | GLY | PRO | GLU | ALA | ALA | ALA | PHE | VAL | LYS | ||||
| 2 | VAL | SER | MET | ASP | GLY | ALA | PRO | TYR | LEU | ARG | ||||
| 3 | LYS | ILE | ASP | LEU | ARG | MET | TYR | LYS | SER | TYR | ||||
| 4 | ASP | GLU | LEU | SER | ASN | ALA | LEU | SER | ASN | MET | ||||
| 5 | PHE | SER | SER | PHE | THR | MET | GLY | LYS | HIS | GLY | ||||
| 6 | GLY | GLU | GLU | GLY | MET | ILE | ASP | PHE | MET | ASN | ||||
| 7 | GLU | ARG | LYS | LEU | MET | ASP | LEU | VAL | ASN | SER | ||||
| 8 | TRP | ASP | TYR | VAL | PRO | SER | TYR | GLU | ASN | LYS | ||||
| 9 | ASP | GLY | ASN | TRP | MET | LEU | VAL | GLY | ASP | VAL | ||||
| 10 | PRO | TRP | PRO | MET | PHE | VAL | ASP | THR | CYS | LYS | ||||
| 11 | ARG | LEU | ARG | LEU | MET | LYS | GLY | SER | ASP | ALA | ||||
| 12 | ILE | GLY | LEU |
Samples:
sample_1: Aux-IAA17, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; DTT 10 mM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 298 K; pH: 7.4; pressure: 1 atm; ionic strength: 0.01 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| GB | AAB70451 AAB84354 AAC39439 AAC39440 AAG53997 |
| REF | NP_171921 XP_002889499 |
| SP | P93830 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts