BMRB Entry 25268
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25268
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Title: SpoVM structure determination
Deposition date: 2014-10-06 Original release date: 2015-03-30
Authors: Tian, Fang; Rill, Richard
Citation: Tian, Fang; Rill, Richard; Castaining, Jean-Philippe; Hsin, Jen; Tan, Irene; Wang, Xingshen; Huang, Kerwyn-Casey; Ramamurthi, Kumaran. "Structural and mechanistic basis for the geometric-driven subcellular localication of a small protein" Nat. Struct. Biol. ., .-..
Assembly members:
Molecule_1, polymer, 26 residues, 3022.7605 Da.
Natural source: Common Name: firmicutes Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Molecule_1: MKFYTIKLPKFLGGIVRAML
GSFRKD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 172 |
| 13C chemical shifts | 89 |
| 15N chemical shifts | 22 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 26 residues - 3022.7605 Da.
| 1 | MET | LYS | PHE | TYR | THR | ILE | LYS | LEU | PRO | LYS | ||||
| 2 | PHE | LEU | GLY | GLY | ILE | VAL | ARG | ALA | MET | LEU | ||||
| 3 | GLY | SER | PHE | ARG | LYS | ASP |
Samples:
sample_1: SpoVM, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; sodium phosphate 16 mM; sodium chloride 80 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 310 K; pH: 6.5; pressure: 1 atm; ionic strength: 80 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker AvanceII 600 MHz
- Bruker AvanceIII 850 MHz
Related Database Links:
| PDB | |
| EMBL | CAA74254 CAB13454 |
| GB | AAC36809 AAU23337 AAU40697 ADM37675 ADV96607 |
| REF | NP_389463 WP_003221545 |
| SP | P37817 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts