BMRB Entry 25293
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25293
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Kalata B7 Ser mutant
Deposition date: 2014-10-22 Original release date: 2015-12-21
Authors: Kristiansen, Per Eugen; Skjeldal, Lars; Goransson, Ulf
Citation: Kristiansen, Per Eugen; Skjeldal, Lars; Goransson, Ulf. "Kalata B7 Ser mutant" To be Published ., .-..
Assembly members:
entity, polymer, 29 residues, 3021.515 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis Host organism: not applicable
Entity Sequences (FASTA):
entity: SWPICKRNGLPVCGETCTLG
TCSTQGCTC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 70 |
| 15N chemical shifts | 22 |
| 1H chemical shifts | 178 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 29 residues - 3021.515 Da.
| 1 | SER | TRP | PRO | ILE | CYS | LYS | ARG | ASN | GLY | LEU | ||||
| 2 | PRO | VAL | CYS | GLY | GLU | THR | CYS | THR | LEU | GLY | ||||
| 3 | THR | CYS | SER | THR | GLN | GLY | CYS | THR | CYS |
Samples:
sample_1: Kalata B7 mutant 1 mM; H2O 5%; D2O, [U-100% 2H], 95%; DSS 0.2 mM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
TOPSPIN v2.4, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts