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BMRB Entry 25343

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25343
MolProbity Validation Chart

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Title: Talin-F3 / RIAM N-terminal Peptide complex

Deposition date: 2014-11-13 Original release date: 2014-12-15

Authors: Yang, Jun; Zhu, Liang; Zhang, Hao; Hirbawi, Jamila; Fukuda, Koichi; Dwivedi, Pallavi; Liu, Jianmin; Byzova, Tatiana; Plow, Edward; Wu, Jinhua; Qin, Jun

Citation: Yang, Jun; Zhu, Liang; Zhang, Hao; Hirbawi, Jamila; Fukuda, Koichi; Dwivedi, Pallavi; Liu, Jianmin; Byzova, Tatiana; Plow, Edward; Wu, Jinhua; Qin, Jun. "Conformational activation of talin by RIAM triggers integrin-mediated cell adhesion"  Nat. Commun. ., .-..

Assembly members:
RIAM-N_peptide, polymer, 24 residues, 2642.966 Da.
Talin-F3, polymer, 93 residues, 10696.451 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis   Host organism: na

Entity Sequences (FASTA):
RIAM-N_peptide: DIDQMFSTLLGEMDLLTQSL GVDT
Talin-F3: YGVSFFLVKEKMKGKNKLVP RLLGITKECVMRVDEKTKEV IQEWSLTNIKRWAASPKSFT LDFGDYQDGYYSVQTTEGEQ IAQLIAGYIDIIL

Data sets:
Data typeCount
1H chemical shifts751
15N chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RIAM-N_peptide1
2Talin-F32

Entities:

Entity 1, RIAM-N_peptide 24 residues - 2642.966 Da.

1   ASPILEASPGLNMETPHESERTHRLEULEU
2   GLYGLUMETASPLEULEUTHRGLNSERLEU
3   GLYVALASPTHR

Entity 2, Talin-F3 93 residues - 10696.451 Da.

1   TYRGLYVALSERPHEPHELEUVALLYSGLU
2   LYSMETLYSGLYLYSASNLYSLEUVALPRO
3   ARGLEULEUGLYILETHRLYSGLUCYSVAL
4   METARGVALASPGLULYSTHRLYSGLUVAL
5   ILEGLNGLUTRPSERLEUTHRASNILELYS
6   ARGTRPALAALASERPROLYSSERPHETHR
7   LEUASPPHEGLYASPTYRGLNASPGLYTYR
8   TYRSERVALGLNTHRTHRGLUGLYGLUGLN
9   ILEALAGLNLEUILEALAGLYTYRILEASP
10   ILEILELEU

Samples:

sample_1: Talin-F3, [U-15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 2 mM; DSS 1 mM; D2O 5%; H2O 95%

sample_2: RIAM-N peptide 1.5 mM; Talin-F3, [U-15N; U-2H], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 2 mM; DSS 1 mM; D2O 5%; H2O 95%

sample_conditions_1: temperature: 273 K; pH: 6.8; pressure: 1 atm; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSY (N/C-filtered)sample_2isotropicsample_conditions_1
2D 1H-1H NOESY (N/C-filtered)sample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis

SPARKY, Goddard - data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton - evaluation

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAA91384 BAC30983 BAC41256 BAE36853 BAG64612 BAA82979 BAC30516 BAC65702 BAE27781 BAG09941
EMBL CAM24322 CAA39588 CDQ87146
GB AAH23110 AAH35636 AAH54516 AAN75525 AIC60178 AAD13152 AAF23322 AAF27330 AAH42923 AAI21760
REF NP_001094047 NP_061916 NP_062329 XP_001101993 XP_003269424 NP_001034114 NP_001192357 NP_006280 NP_035732 NP_989854
SP Q7Z5R6 Q8R5A3 P26039 P54939 Q9Y490
BMRB 15792 16932
PRF 1617167A
TPG DAA26829

Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone or all simulated shifts