BMRB Entry 25352
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25352
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Title: Acidocin B PubMed: 25681186
Deposition date: 2014-11-19 Original release date: 2015-03-02
Authors: Vederas, John; Acedo, Jeella; van Belkum, Marco; Lohans, Christopher
Citation: Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; McKay, R.; Miskolzie, M.; Vederas, John. "Solution Structure of Acidocin B, a Circular Bacteriocin Produced by Lactobacillus acidophilus M46" Appl. Environ. Microbiol. 81, 2910-2918 (2015).
Assembly members:
entity, polymer, 58 residues, 5642.548 Da.
Natural source: Common Name: lactic acid bacteria Taxonomy ID: 1579 Superkingdom: Bacteria Kingdom: not available Genus/species: Lactobacillus acidophilus
Experimental source: Production method: purified from the natural source Host organism: Lactobacillus acidophilus
Entity Sequences (FASTA):
entity: IYWIADQFGIHLATGTARKL
LDAVASGASLGTAFAAILGV
TLPAWALAAAGALGATAA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 377 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 58 residues - 5642.548 Da.
N- and C-termini are cyclized
| 1 | ILE | TYR | TRP | ILE | ALA | ASP | GLN | PHE | GLY | ILE | ||||
| 2 | HIS | LEU | ALA | THR | GLY | THR | ALA | ARG | LYS | LEU | ||||
| 3 | LEU | ASP | ALA | VAL | ALA | SER | GLY | ALA | SER | LEU | ||||
| 4 | GLY | THR | ALA | PHE | ALA | ALA | ILE | LEU | GLY | VAL | ||||
| 5 | THR | LEU | PRO | ALA | TRP | ALA | LEU | ALA | ALA | ALA | ||||
| 6 | GLY | ALA | LEU | GLY | ALA | THR | ALA | ALA |
Samples:
sample_1: entity 1 mM; SDS, [U-99% 2H], 80 mM; DSS 0.5 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Agilent vNMRS 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA22261 BAH08712 |
| EMBL | CAA84399 |
| GB | AJP07153 |
| PRF | 2120355B |
| REF | WP_012621083 YP_002474143 |