BMRB Entry 25404
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25404
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Title: Solution structure of VPg of porcine sapovirus PubMed: 25753201
Deposition date: 2014-12-24 Original release date: 2015-04-13
Authors: Kim, Jeong-Sun; Hwang, Hyo-Jeong; Min, Hye Jung; Yun, Hyosuk; Cho, Kyoung-Oh; Pelton, Jeffrey; Wemmer, David; Lee, Chul Won
Citation: Hwang, Hyo-Jeong; Min, Hye Jung; Yun, Hyosuk; Pelton, Jeffrey; Wemmer, David; Cho, Kyoung-Oh; Kim, Jeong-Sun; Lee, Chul Won. "Solution structure of the porcine sapovirus VPg core reveals a stable three-helical bundle with a conserved surface patch" Biochem. Biophys. Res. Commun. 459, 610-616 (2015).
Assembly members:
entity, polymer, 62 residues, 7382.292 Da.
Natural source: Common Name: PEDV Taxonomy ID: 28295 Superkingdom: Viruses Kingdom: not available Genus/species: Alphacoronavirus Porcine epidemic diarrhea virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GAMAIALRDDEYDEWQDIIR
DWRKEMTVQQFLDLKERALS
GASDPDSQRYNAWLELRAKR
LS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 277 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 443 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 62 residues - 7382.292 Da.
| 1 | GLY | ALA | MET | ALA | ILE | ALA | LEU | ARG | ASP | ASP | ||||
| 2 | GLU | TYR | ASP | GLU | TRP | GLN | ASP | ILE | ILE | ARG | ||||
| 3 | ASP | TRP | ARG | LYS | GLU | MET | THR | VAL | GLN | GLN | ||||
| 4 | PHE | LEU | ASP | LEU | LYS | GLU | ARG | ALA | LEU | SER | ||||
| 5 | GLY | ALA | SER | ASP | PRO | ASP | SER | GLN | ARG | TYR | ||||
| 6 | ASN | ALA | TRP | LEU | GLU | LEU | ARG | ALA | LYS | ARG | ||||
| 7 | LEU | SER |
Samples:
sample_1: entity, [U-99% 13C; U-99% 15N], 1 mM; potassium phosphate 10 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 10 mM; pH: 6.2; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts