BMRB Entry 25433
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25433
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Title: NMR structure of the first Zinc Finger domain of RBM10
Deposition date: 2015-01-16 Original release date: 2015-01-26
Authors: Serrano, Pedro; Wuthrich, Kurt; Geralt, Michael
Citation: Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the first Zinc Finger domain of RBM10" Not known ., .-..
Assembly members:
entity_1, polymer, 43 residues, 4957.872 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GHMPKINEDWLCNKCGVQNF
KRREKCFKCGVPKSEAEQKL
PLG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 228 |
| 13C chemical shifts | 140 |
| 15N chemical shifts | 39 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, entity_1 43 residues - 4957.872 Da.
| 1 | GLY | HIS | MET | PRO | LYS | ILE | ASN | GLU | ASP | TRP | ||||
| 2 | LEU | CYS | ASN | LYS | CYS | GLY | VAL | GLN | ASN | PHE | ||||
| 3 | LYS | ARG | ARG | GLU | LYS | CYS | PHE | LYS | CYS | GLY | ||||
| 4 | VAL | PRO | LYS | SER | GLU | ALA | GLU | GLN | LYS | LEU | ||||
| 5 | PRO | LEU | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: entity, [U-99% 13C; U-98% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 298 K; pH: 6.0; pressure: 1 atm; ionic strength: 0.0798 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| APSY 4D-HACANH | sample_1 | isotropic | sample_conditions_1 |
| APSY 5D-HACACONH | sample_1 | isotropic | sample_conditions_1 |
| APSY 5D-CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CNS, Brunger A. T. et.al., Herrman & Wuthrich - refinement, chemical shift assignment, peak picking, structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 17386 |
| PDB | |
| DBJ | BAA09471 BAA12144 BAC31087 BAC40753 BAC65490 |
| EMBL | CAD97933 CAL37951 |
| GB | AAH00681 AAH03089 AAH04181 AAH04674 AAH08733 |
| REF | NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397 |
| SP | P70501 P98175 Q99KG3 |
| TPG | DAA12902 DAA12903 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts