BMRB Entry 25529
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25529
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Title: NMR Solution Structure and Model Membrane Interaction Studies of the Peptide Hylin a1 from the Arboreal South American Frog Hypsiboas albopunctatus PubMed: 26059694
Deposition date: 2015-03-11 Original release date: 2015-06-22
Authors: Alves, Eliane; Oliveira, Aline; Alves, Eliane
Citation: Alves, Eliane; Junior, Edson; Cilli, Eduardo; Castro, Mariana; Fontes, Wagner; Magalhaes, Mariana; Liao, Luciano; Oliveira, Aline. "Micelle Bound Structure and Model Membrane Interaction Studies of the Peptide Hylin a1 from the Arboreal South American Frog Hypsiboas albopunctatus" Protein Pept. Lett. 22, 719-726 (2015).
Assembly members:
entity, polymer, 18 residues, 1867.386 Da.
Natural source: Common Name: Spotted tree frog Taxonomy ID: 279985 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hypsiboas albopunctatus
Experimental source: Production method: obtained from a collaborator Host organism: Hypsiboas albopunctatus
Entity Sequences (FASTA):
entity: IFGAILPLALGALKNLIK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 123 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 18 residues - 1867.386 Da.
| 1 | ILE | PHE | GLY | ALA | ILE | LEU | PRO | LEU | ALA | LEU | ||||
| 2 | GLY | ALA | LEU | LYS | ASN | LEU | ILE | LYS |
Samples:
sample_1: Hylin a1 1 mM; SDS-d25, [U-2H], 100 mM; D2O, [U-99% 2H], 10%; H2O 90%; TMSP 1 mg/uL
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH v2.28, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
ProcheckNMR, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton - data analysis
Molmol, Koradi, Billeter and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz