BMRB Entry 25544
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25544
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Title: Solution structure of human Myosin VI isoform3 (1050-1131) PubMed: 26971995
Deposition date: 2015-03-20 Original release date: 2016-03-07
Authors: He, Fahu; Walters, Kylie
Citation: He, Fahu; Wollscheid, Hans-Peter; Nowicka, Urszula; Biancospino, Matteo; Valentini, Eleonora; Ehlinger, Aaron; Acconcia, Filippo; Magistrati, Elisa; Polo, Simona; Walters, Kylie. "Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains" Cell Rep. 14, 2683-2694 (2016).
Assembly members:
entity, polymer, 82 residues, 9580.269 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: RPKMTPEQMAKEMSEFLSRG
PAVLATKAAAGTKKYDLSKW
KYAELRDTINTSCDIELLAA
CREEFHRRLKVYHAWKSKNK
KR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 366 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 607 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 82 residues - 9580.269 Da.
| 1 | ARG | PRO | LYS | MET | THR | PRO | GLU | GLN | MET | ALA | ||||
| 2 | LYS | GLU | MET | SER | GLU | PHE | LEU | SER | ARG | GLY | ||||
| 3 | PRO | ALA | VAL | LEU | ALA | THR | LYS | ALA | ALA | ALA | ||||
| 4 | GLY | THR | LYS | LYS | TYR | ASP | LEU | SER | LYS | TRP | ||||
| 5 | LYS | TYR | ALA | GLU | LEU | ARG | ASP | THR | ILE | ASN | ||||
| 6 | THR | SER | CYS | ASP | ILE | GLU | LEU | LEU | ALA | ALA | ||||
| 7 | CYS | ARG | GLU | GLU | PHE | HIS | ARG | ARG | LEU | LYS | ||||
| 8 | VAL | TYR | HIS | ALA | TRP | LYS | SER | LYS | ASN | LYS | ||||
| 9 | LYS | ARG |
Samples:
sample_1: entity, [U-13C; U-15N], 0.7 mM; sodium phosphate buffer 20 mM; NaCl 50 mM; DTT 2 mM; NaN3 0.1%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Kujira, Naohiro Kobayashi - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts