BMRB Entry 25552
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25552
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Title: STIL binding to Polo-box 3 of PLK4 regulates centriole duplication - NMR solution structure of human Polo-box 3 PubMed: 26188084
Deposition date: 2015-03-24 Original release date: 2015-08-07
Authors: Boehm, Raphael; Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Sauer, Evelyn; Nigg, Erich; Maier, Timm; Hiller, Sebastian
Citation: Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Boehm, Raphael; Sauer, Evelyn; Hiller, Sebastian; Nigg, Erich; Maier, Timm. "STIL binding to Polo-box 3 of PLK4 regulates centriole duplication." eLife 4, .-. (2015).
Assembly members:
entity, polymer, 88 residues, 9629.920 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GSAQLLKSVFVKNVGWATQL
TSGAVWVQFNDGSQLVVQAG
VSSISYTSPNGQTTRYGENE
KLPDYIKQKLQCLSSILLMF
SNPTPNFH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 266 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 192 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 88 residues - 9629.920 Da.
| 1 | GLY | SER | ALA | GLN | LEU | LEU | LYS | SER | VAL | PHE | ||||
| 2 | VAL | LYS | ASN | VAL | GLY | TRP | ALA | THR | GLN | LEU | ||||
| 3 | THR | SER | GLY | ALA | VAL | TRP | VAL | GLN | PHE | ASN | ||||
| 4 | ASP | GLY | SER | GLN | LEU | VAL | VAL | GLN | ALA | GLY | ||||
| 5 | VAL | SER | SER | ILE | SER | TYR | THR | SER | PRO | ASN | ||||
| 6 | GLY | GLN | THR | THR | ARG | TYR | GLY | GLU | ASN | GLU | ||||
| 7 | LYS | LEU | PRO | ASP | TYR | ILE | LYS | GLN | LYS | LEU | ||||
| 8 | GLN | CYS | LEU | SER | SER | ILE | LEU | LEU | MET | PHE | ||||
| 9 | SER | ASN | PRO | THR | PRO | ASN | PHE | HIS |
Samples:
Deuterated-triple_labelled_PB3: PLK4-PB3, [U-2H; U-13C; U-15N], 0.6 – 1.2 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%
ILV_PB3_NOESY-type: PLK4-PB3, [U-2H; U-15N; ILV(methyl)-13C,1H], 0.6 – 1.2 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%
ILV_PB3_TOCSY-type: PLK4-PB3, [U-2H; U-13C; U-15N; ILV(methyl)-1H], 1.2 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%
Deuterated-15N_PB3: PLK4-PB3, [U-2H; U-15N], 0.6 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%
sample_conditions_1: ionic strength: 30 mM; pH: 7; pressure: 1 atm; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | Deuterated-triple_labelled_PB3 | isotropic | sample_conditions_1 |
| 3D HNCO | Deuterated-triple_labelled_PB3 | isotropic | sample_conditions_1 |
| 3D HNCA | Deuterated-triple_labelled_PB3 | isotropic | sample_conditions_1 |
| 3D HNCACB | Deuterated-triple_labelled_PB3 | isotropic | sample_conditions_1 |
| 3D HNCACO | Deuterated-triple_labelled_PB3 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | ILV_PB3_NOESY-type | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | ILV_PB3_NOESY-type | isotropic | sample_conditions_1 |
| 3D (H)C(CC)-TOCSY-(CO)-15N-HSQC | ILV_PB3_TOCSY-type | isotropic | sample_conditions_1 |
| 3D H(C)(CC)-TOCSY-(CO)-15N-HSQC | ILV_PB3_TOCSY-type | isotropic | sample_conditions_1 |
| 15N-filtered BPP-LED NMR | Deuterated-15N_PB3 | isotropic | sample_conditions_1 |
| 15N-{1H}-Heteronuclear NOE | Deuterated-15N_PB3 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | ILV_PB3_TOCSY-type | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, data analysis, peak picking, structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 26547 |
| PDB | |
| DBJ | BAB24599 BAB69958 BAE02419 BAE23231 BAE23367 |
| EMBL | CAA73575 CAH91413 |
| GB | AAC37648 AAH26785 AAH36023 AAH57940 AAX43264 |
| REF | NP_001153257 NP_001177728 NP_001177730 NP_035625 NP_055079 |
| SP | O00444 Q5R9Z7 Q64702 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts