BMRB Entry 25699
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25699
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Title: Structure of an N-terminal membrane-anchoring region of the glycosyltransferase WaaG PubMed: 26244737
Deposition date: 2015-07-13 Original release date: 2015-08-17
Authors: Liebau, Jobst; Pettersson, Pontus; Szpryngiel, Scarlett; Maler, Lena
Citation: Liebau, Jobst; Pettersson, Pontus; Szpryngiel, Scarlett; Maler, Lena. "Membrane Interaction of the Glycosyltransferase WaaG" Biophys. J. 109, 552-563 (2015).
Assembly members:
entity, polymer, 30 residues, 3680.196 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: YAEKVAQEKGFLYRLTSRYR
HYAAFERATF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 84 |
| 1H chemical shifts | 219 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 30 residues - 3680.196 Da.
| 1 | TYR | ALA | GLU | LYS | VAL | ALA | GLN | GLU | LYS | GLY | |
| 2 | PHE | LEU | TYR | ARG | LEU | THR | SER | ARG | TYR | ARG | |
| 3 | HIS | TYR | ALA | ALA | PHE | GLU | ARG | ALA | THR | PHE |
Samples:
sample_1: entity 0.6 mM; DPC, [U-100% 2H], 50 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz