BMRB Entry 25761
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25761
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Title: NMR structure of a DUF1491 family protein (CC_1065) from Caulobacter crescentus CB15
Deposition date: 2015-08-19 Original release date: 2015-10-12
Authors: Qin, Haina; Serrano, Pedro; Wuthrich, Kurt
Citation: Qin, Haina; Wuthrich, Kurt; Serrano, Pedro. "NMR structure of a DUF1491 family protein (CC_1065) from Caulobacter crescentus CB15" to be published ., .-..
Assembly members:
entity, polymer, 110 residues, 12262.887 Da.
Natural source: Common Name: a-proteobacteria Taxonomy ID: 190650 Superkingdom: Bacteria Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GMLLSTDIWVAALIRRAELG
GAFATVARKGDARAGAVLVK
AVDRREGTARLFSEATRGDG
ERFWMQPVRSTFEPDLDAYA
ERAARIDPDIWVVEIEDRDG
RHFLTEPVES
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 355 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 715 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 110 residues - 12262.887 Da.
| 1 | GLY | MET | LEU | LEU | SER | THR | ASP | ILE | TRP | VAL | |
| 2 | ALA | ALA | LEU | ILE | ARG | ARG | ALA | GLU | LEU | GLY | |
| 3 | GLY | ALA | PHE | ALA | THR | VAL | ALA | ARG | LYS | GLY | |
| 4 | ASP | ALA | ARG | ALA | GLY | ALA | VAL | LEU | VAL | LYS | |
| 5 | ALA | VAL | ASP | ARG | ARG | GLU | GLY | THR | ALA | ARG | |
| 6 | LEU | PHE | SER | GLU | ALA | THR | ARG | GLY | ASP | GLY | |
| 7 | GLU | ARG | PHE | TRP | MET | GLN | PRO | VAL | ARG | SER | |
| 8 | THR | PHE | GLU | PRO | ASP | LEU | ASP | ALA | TYR | ALA | |
| 9 | GLU | ARG | ALA | ALA | ARG | ILE | ASP | PRO | ASP | ILE | |
| 10 | TRP | VAL | VAL | GLU | ILE | GLU | ASP | ARG | ASP | GLY | |
| 11 | ARG | HIS | PHE | LEU | THR | GLU | PRO | VAL | GLU | SER |
Samples:
sample_1: entity, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.240 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| APSY 4D-HACANH | sample_1 | isotropic | sample_conditions_1 |
| APSY 5D-CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| APSY 5D-HACACONH | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, G??ntert P. - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts