BMRB Entry 25803
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25803
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Title: UBL domain of the yeast DNA damage-inducible protein homolog 1 PubMed: 27009356
Deposition date: 2015-09-09 Original release date: 2016-07-13
Authors: Siva, Monika; Grantz Saskova, Klara; Veverka, Vaclav
Citation: Klima, Martin; Toth, Daniel; Hexnerova, Rozalie; Baumlova, Adriana; Chalupska, Dominika; Tykvart, Jan; Rezabkova, Lenka; Sengupta, Nivedita; Man, Petr; Dubankova, Anna; Humpolickova, Jana; Nencka, Radim; Veverka, Vaclav; Balla, Tamas; Boura, Evzen. "Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein" Sci. Rep. 6, 23641-23641 (2016).
Assembly members:
entity, polymer, 86 residues, 9692.008 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: HIEGRHMDLTISNELTGEIY
GPIEVSEDMALTDLIALLQA
DCGFDKTKHDLYYNMDILDS
NRTQSLKELGLKTDDLLLIR
GKISNS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 360 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 614 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 86 residues - 9692.008 Da.
| 1 | HIS | ILE | GLU | GLY | ARG | HIS | MET | ASP | LEU | THR | ||||
| 2 | ILE | SER | ASN | GLU | LEU | THR | GLY | GLU | ILE | TYR | ||||
| 3 | GLY | PRO | ILE | GLU | VAL | SER | GLU | ASP | MET | ALA | ||||
| 4 | LEU | THR | ASP | LEU | ILE | ALA | LEU | LEU | GLN | ALA | ||||
| 5 | ASP | CYS | GLY | PHE | ASP | LYS | THR | LYS | HIS | ASP | ||||
| 6 | LEU | TYR | TYR | ASN | MET | ASP | ILE | LEU | ASP | SER | ||||
| 7 | ASN | ARG | THR | GLN | SER | LEU | LYS | GLU | LEU | GLY | ||||
| 8 | LEU | LYS | THR | ASP | ASP | LEU | LEU | LEU | ILE | ARG | ||||
| 9 | GLY | LYS | ILE | SER | ASN | SER |
Samples:
sample_1: sodium phosphate 25 mM; sodium chloride 100 mM; entity, [U-13C; U-15N], 0.4 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
YASARA, YASARA - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts