BMRB Entry 25817
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25817
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Title: Structure of the transmembrane domain of human nicastrin in SDS micelles PubMed: 26776682
Deposition date: 2015-09-17 Original release date: 2016-04-25
Authors: Li, Yan; Liew, Lynette; Li, Qingxin; Kang, CongBao
Citation: Li, Yan; Liew, Lynette; Li, Qingxin; Kang, CongBao. "Structure of the transmembrane domain of human nicastrin-a component of -secretase" Sci. Rep. 6, 19522-19522 (2016).
Assembly members:
transmembrane_domain_of_human_nicastrin, polymer, 54 residues, 4960.896 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
transmembrane_domain_of_human_nicastrin: MAHHHHHHASKELELITLTV
GFGILIFSLIVTYCINAKAD
VLFIAPREPGAVSY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 128 |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 262 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | transmembrane_domain_of_human_nicastrin | 1 |
Entities:
Entity 1, transmembrane_domain_of_human_nicastrin 54 residues - 4960.896 Da.
| 1 | MET | ALA | HIS | HIS | HIS | HIS | HIS | HIS | ALA | SER | ||||
| 2 | LYS | GLU | LEU | GLU | LEU | ILE | THR | LEU | THR | VAL | ||||
| 3 | GLY | PHE | GLY | ILE | LEU | ILE | PHE | SER | LEU | ILE | ||||
| 4 | VAL | THR | TYR | CYS | ILE | ASN | ALA | LYS | ALA | ASP | ||||
| 5 | VAL | LEU | PHE | ILE | ALA | PRO | ARG | GLU | PRO | GLY | ||||
| 6 | ALA | VAL | SER | TYR |
Samples:
sample_1: transmembrane domain of human nicastrin, [U-100% 13C; U-100% 15N], 0.8 mM; sodium phosphate 20 mM; SDS 100 mM; DTT 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Keller and Wuthrich, Schwieters, Kuszewski, Tjandra and Clore - chemical shift assignment, processing, structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts