BMRB Entry 25929

Name: Transmembrane domain of mouse Fas/CD95 death receptor
Published: 2016-01-25

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PDB ID: 2na6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25929
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Transmembrane domain of mouse Fas/CD95 death receptor PubMed: 26853147

Deposition date: 2015-12-21 Original release date: 2016-01-25

Authors: Fu, Qingshan; Chou, James; Wu, Hao; Fu, Tianmin

Citation: Fu, Qingshan; Fu, Tianmin; Cruz, Anthony; Sengupta, Prabuddha; Thomas, Stacy; Wang, Shuqing; Siegel, Richard; Wu, Hao; Chou, James. "Structural Basis and Functional Role of Intramembrane Trimerization of the Fas/CD95 Death Receptor" Mol. Cell 61, 602-613 (2016).

Assembly members:
Mouse_Fas_Transmembrane_Domain, polymer, 28 residues, 3580.532 Da.

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Mouse_Fas_Transmembrane_Domain: RNRLWLLTILVLLIPLVFIY RKYRKRKS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	52
15N chemical shifts	25
1H chemical shifts	25

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	1
3	entity_3	1

Entities:

Entity 1, entity_1 28 residues - 3580.532 Da.

1

ARG

ASN

ARG

LEU

TRP

LEU

THR

ILE

LEU

2

VAL

LEU

ILE

PRO

LEU

VAL

PHE

ILE

TYR

3

ARG

LYS

TYR

ARG

LYS

ARG

LYS

SER

Samples:

NC-labeled: Mouse Fas Transmembrane Domain, [U-100% 13C; U-100% 15N], 1 ± 0.05 mM; DMPC, [U-100% 2H] acyl chains, 60 ± 1 mM; DHPC, [U-100% 2H] acyl chains, 120 ± 5 mM; sodium phosphate 20 ± 1 mM; H2O 95%; D2O 5%

NCD-labeled: Mouse Fas Transmembrane Domain, [U-100% 13C; U-100% 15N; U-85% 2H], 1 ± 0.05 mM; DMPC 60 ± 1 mM; DHPC 120 ± 5 mM; sodium phosphate 20 ± 1 mM; H2O 95%; D2O 5%

Mix-labeled: Mouse Fas Transmembrane Domain, [U-100% 15N; U-100% 2H], 0.5 ± 0.05 mM; Mouse Fas Transmembrane Domain, [U-15% 13C], 0.5 ± 0.05 mM; DMPC, [U-100% 2H] acyl chains, 60 ± 1 mM; DHPC, [U-100% 2H] acyl chains, 120 ± 5 mM; sodium phosphate 20 ± 1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY HSQC	NCD-labeled	isotropic	sample_conditions_1
3D TROSY HNCA	NCD-labeled	isotropic	sample_conditions_1
3D TROSY HN(CO)CA	NCD-labeled	isotropic	sample_conditions_1
3D TROSY HN(CA)CO	NCD-labeled	isotropic	sample_conditions_1
3D TROSY HNCO	NCD-labeled	isotropic	sample_conditions_1
3D 1H-15N NOESY	NC-labeled	isotropic	sample_conditions_1
3D 1H-13C NOESY	NC-labeled	isotropic	sample_conditions_1
3D 1H-15N NOESY	Mix-labeled	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	Mix-labeled	isotropic	sample_conditions_1
2D 1H-15N TROSY HSQC	Mix-labeled	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

XEASY, Bartels et al. - chemical shift assignment, data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts