BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25947

Title: Solution structure of KYE28A in lipopolysachharide   PubMed: 27137928

Deposition date: 2016-01-11 Original release date: 2016-05-23

Authors: Bhunia, Anirban; Malmsten, Martin; Datta, Aritreyee

Citation: Bhunia, Anirban; Malmsten, Martin; Datta, Aritreyee. "Role of Aromatic Amino Acids in Lipopolysaccharide and Membrane Interactions of Antimicrobial Peptides for Use in Plant Disease Control"  J. Biol. Chem. 291, 13301-13317 (2016).

Assembly members:
entity, polymer, 28 residues, 3283.888 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: KYEITTIHNLARKLTHRLAR RNAGATLR

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 28 residues - 3283.888 Da.

1   LYSTYRGLUILETHRTHRILEHISASNLEU
2   ALAARGLYSLEUTHRHISARGLEUALAARG
3   ARGASNALAGLYALATHRLEUARG

Samples:

sample_1: entity 1 mM; TSP 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Ascend TM 700 MHz