BMRB Entry 25975
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25975
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Title: Structure and Dynamics of the Geobacillus stearothermophilus IF2 G3-subdomain PubMed: 27364543
Deposition date: 2016-02-11 Original release date: 2016-07-18
Authors: Dongre, Ramachandra; Folkers, Gert; Gualerzi, Claudio; Boelens, Rolf; Wienk, Hans
Citation: Dongre, Ramachandra; Folkers, Gert; Gualerzi, Claudio; Boelens, Rolf; Wienk, Hans. "A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit" Protein Sci. 25, 1722-1733 (2016).
Assembly members:
entity, polymer, 125 residues, 13649.863 Da.
Natural source: Common Name: fermicutes Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: Terrabacteria Genus/species: Bacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GSHMLDHLLEMILLVSEMEE
LKANPNRRAVGTVIEAKLDK
GRGPVATLLVQAGTLKVGDP
IVVGTTYGRVRAMVNDSGRR
VKEAGPSMPVEITGLHDVPQ
AGDRFMVFEDEKKARQIGEA
RAQRQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 402 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 653 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 125 residues - 13649.863 Da.
| 1 | GLY | SER | HIS | MET | LEU | ASP | HIS | LEU | LEU | GLU | ||||
| 2 | MET | ILE | LEU | LEU | VAL | SER | GLU | MET | GLU | GLU | ||||
| 3 | LEU | LYS | ALA | ASN | PRO | ASN | ARG | ARG | ALA | VAL | ||||
| 4 | GLY | THR | VAL | ILE | GLU | ALA | LYS | LEU | ASP | LYS | ||||
| 5 | GLY | ARG | GLY | PRO | VAL | ALA | THR | LEU | LEU | VAL | ||||
| 6 | GLN | ALA | GLY | THR | LEU | LYS | VAL | GLY | ASP | PRO | ||||
| 7 | ILE | VAL | VAL | GLY | THR | THR | TYR | GLY | ARG | VAL | ||||
| 8 | ARG | ALA | MET | VAL | ASN | ASP | SER | GLY | ARG | ARG | ||||
| 9 | VAL | LYS | GLU | ALA | GLY | PRO | SER | MET | PRO | VAL | ||||
| 10 | GLU | ILE | THR | GLY | LEU | HIS | ASP | VAL | PRO | GLN | ||||
| 11 | ALA | GLY | ASP | ARG | PHE | MET | VAL | PHE | GLU | ASP | ||||
| 12 | GLU | LYS | LYS | ALA | ARG | GLN | ILE | GLY | GLU | ALA | ||||
| 13 | ARG | ALA | GLN | ARG | GLN |
Samples:
sample_1: IF2-G3, [U-100% 13C; U-100% 15N], 0.15 mM; sodium phosphate 50 mM; potassium chloride 100 mM; glycerol 2%; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
TALOS, Cornilescu, Delaglio and Bax - data analysis
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts