BMRB Entry 26649
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR26649
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Title: 1H, 13C, 15N chemical shift assignments of initiation factor 1 from Pseudomonas aeruginosa PubMed: 26983940
Deposition date: 2015-09-04 Original release date: 2016-08-22
Authors: Zhang, Yonghong
Citation: Bernal, Alejandra; Hu, Yanmei; Palmer, Stephanie; Silva, Aaron; Bullard, James; Zhang, Yonghong. "1H, 13C and 15N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa" Biomol NMR Assign. 10, 249-252 (2016).
Assembly members:
Initiation_Factor_IF-1, polymer, 80 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Initiation_Factor_IF-1: MSKEDSFEMEGTVVDTLPNT
MFRVELENGHVVTAHISGKM
RKNYIRILTGDKVRVELTPY
DLSKGRITYRARLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 269 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 460 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IF1 | 1 |
Entities:
Entity 1, IF1 80 residues - Formula weight is not available
| 1 | MET | SER | LYS | GLU | ASP | SER | PHE | GLU | MET | GLU | |
| 2 | GLY | THR | VAL | VAL | ASP | THR | LEU | PRO | ASN | THR | |
| 3 | MET | PHE | ARG | VAL | GLU | LEU | GLU | ASN | GLY | HIS | |
| 4 | VAL | VAL | THR | ALA | HIS | ILE | SER | GLY | LYS | MET | |
| 5 | ARG | LYS | ASN | TYR | ILE | ARG | ILE | LEU | THR | GLY | |
| 6 | ASP | LYS | VAL | ARG | VAL | GLU | LEU | THR | PRO | TYR | |
| 7 | ASP | LEU | SER | LYS | GLY | ARG | ILE | THR | TYR | ARG | |
| 8 | ALA | ARG | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Initiation Factor IF-1, [U-100% 13C; U-100% 15N], 1 mM; KPO4 25 mM; KCl 100 mM
sample_conditions_1: ionic strength: 0.125 M; pH: 5.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
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