BMRB Entry 26844
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26844
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Title: NMR assignments for the insertion domain of bacteriophage Sf6 coat protein PubMed: 27798771
Deposition date: 2016-07-05 Original release date: 2018-10-03
Authors: Tripler, Therese; Teschke, Carolyn; Alexandrescu, Andrei
Citation: Tripler, Therese; Teschke, Carolyn; Alexandresu, Andrei. "NMR assignments for the insertion domain of bacteriophage Sf6 coat protein" Biomol. NMR Assignments 11, 35-38 (2017).
Assembly members:
Sf6id, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: Sf6 Bacteriophage Taxonomy ID: 38018 Superkingdom: Virsues Kingdom: not available Genus/species: Sf6 Bacteriophage
Experimental source: Production method: recombinant technology Host organism: Shigella flexneri
Entity Sequences (FASTA):
Sf6id: GAFGGTLTVKTQPTVTYNAV
KDSYQFTVTLTGATASVTGF
LKAGDQVKFTNTYWLQQQTK
QALYNGATPISFTATVTADA
NSDSGGDVTVTLSGVPIYDT
TNPQYNSVSRQVEAGDAVSV
VGTA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 448 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 754 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sf6id | 1 |
Entities:
Entity 1, Sf6id 124 residues - Formula weight is not available
contains C-terminal 6x his-tag
| 1 | GLY | ALA | PHE | GLY | GLY | THR | LEU | THR | VAL | LYS | ||||
| 2 | THR | GLN | PRO | THR | VAL | THR | TYR | ASN | ALA | VAL | ||||
| 3 | LYS | ASP | SER | TYR | GLN | PHE | THR | VAL | THR | LEU | ||||
| 4 | THR | GLY | ALA | THR | ALA | SER | VAL | THR | GLY | PHE | ||||
| 5 | LEU | LYS | ALA | GLY | ASP | GLN | VAL | LYS | PHE | THR | ||||
| 6 | ASN | THR | TYR | TRP | LEU | GLN | GLN | GLN | THR | LYS | ||||
| 7 | GLN | ALA | LEU | TYR | ASN | GLY | ALA | THR | PRO | ILE | ||||
| 8 | SER | PHE | THR | ALA | THR | VAL | THR | ALA | ASP | ALA | ||||
| 9 | ASN | SER | ASP | SER | GLY | GLY | ASP | VAL | THR | VAL | ||||
| 10 | THR | LEU | SER | GLY | VAL | PRO | ILE | TYR | ASP | THR | ||||
| 11 | THR | ASN | PRO | GLN | TYR | ASN | SER | VAL | SER | ARG | ||||
| 12 | GLN | VAL | GLU | ALA | GLY | ASP | ALA | VAL | SER | VAL | ||||
| 13 | VAL | GLY | THR | ALA |
Samples:
sample_1: insertion domain, [U-100% 15N], 1.5 mM; insertion domain, [U-100% 13C; U-100% 15N], 2.1 mM; H2O 90%; D2O 10%
sample_2: insertion domain, [U-100% 13C; U-100% 15N], 2.1 mM; D2O 100%
sample_conditions_1: ionic strength: 20 mM; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC NH2 only | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
Ccpnmr_analysis, CCPN - chemical shift assignment, peak picking
Felix, Accelrys Software Inc. - processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts