BMRB Entry 27085
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27085
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Title: 1H, 13C, 15N backbone NMR assignment of N-terminal domain of SaHPF PubMed: 28980143
Deposition date: 2017-04-27 Original release date: 2017-10-05
Authors: Usachev, Konstantin; Ayupov, Rustam; Validov, Shamil; Khusainov, Iskander; Yusupov, Marat
Citation: Usachev, Konstantin; Ayupov, Rustam; Validov, Shamil; Khusainov, Iskander; Yusupov, Marat. "NMR assignments of the N-terminal domain of Staphylococcus aureus hibernation promoting factor (SaHPF)" Biomol. NMR Assignments 12, 85-89 (2018).
Assembly members:
SaHPF_NTD, polymer, 110 residues, 12913.7078 Da.
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SaHPF_NTD: MIRFEIHGDNLTITDAIRNY
IEEKIGKLERYFNDVPNAVA
HVKVKTYSNSATKIEVTIPL
KNVTLRAEERNDDLYAGIDL
INNKLERQVRKYKTRINRKS
RDRGDQEVFV
| Data type | Count |
| 13C chemical shifts | 435 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 501 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SaHPF NTD | 1 |
Entities:
Entity 1, SaHPF NTD 110 residues - 12913.7078 Da.
| 1 | MET | ILE | ARG | PHE | GLU | ILE | HIS | GLY | ASP | ASN | |
| 2 | LEU | THR | ILE | THR | ASP | ALA | ILE | ARG | ASN | TYR | |
| 3 | ILE | GLU | GLU | LYS | ILE | GLY | LYS | LEU | GLU | ARG | |
| 4 | TYR | PHE | ASN | ASP | VAL | PRO | ASN | ALA | VAL | ALA | |
| 5 | HIS | VAL | LYS | VAL | LYS | THR | TYR | SER | ASN | SER | |
| 6 | ALA | THR | LYS | ILE | GLU | VAL | THR | ILE | PRO | LEU | |
| 7 | LYS | ASN | VAL | THR | LEU | ARG | ALA | GLU | GLU | ARG | |
| 8 | ASN | ASP | ASP | LEU | TYR | ALA | GLY | ILE | ASP | LEU | |
| 9 | ILE | ASN | ASN | LYS | LEU | GLU | ARG | GLN | VAL | ARG | |
| 10 | LYS | TYR | LYS | THR | ARG | ILE | ASN | ARG | LYS | SER | |
| 11 | ARG | ASP | ARG | GLY | ASP | GLN | GLU | VAL | PHE | VAL |
Samples:
NTD-SaHPF: SaHPF NTD, [U-100% 13C; U-100% 15N], 0.62 mM; NH4Cl 200.00 mM
condition1: ionic strength: 0.200 M; pH: 7.600; pressure: 1.000 atm; temperature: 308.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | NTD-SaHPF | isotropic | condition1 |
| 2D 1H-13C HSQC/HMQC | NTD-SaHPF | isotropic | condition1 |
| 2D 1H-13C HSQC/HMQC | NTD-SaHPF | isotropic | condition1 |
| 3D HNCA | NTD-SaHPF | isotropic | condition1 |
| 3D CBCA(CO)NH | NTD-SaHPF | isotropic | condition1 |
| 3D HNCACB | NTD-SaHPF | isotropic | condition1 |
| 3D HNCO | NTD-SaHPF | isotropic | condition1 |
| HN(CA)CO (H[N[ca[CO]]]) | NTD-SaHPF | isotropic | condition1 |
| CC(CO)NH (C_coNH.relayed) | NTD-SaHPF | isotropic | condition1 |
| 3D H(CCO)NH | NTD-SaHPF | isotropic | condition1 |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts