BMRB Entry 27552
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27552
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Title: Chemical Shift Assignments for m62A-h45
Deposition date: 2018-07-18 Original release date: 2019-06-03
Authors: Liu, Xiaodan
Citation: Liu, Xiaodan. "Assigned Chemical Shifts for m62A-h45" Not known ., .-..
Assembly members:
m62A-h45, polymer, 24 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: Escherichia coli
Entity Sequences (FASTA):
m62A-h45: UAAGUGUACUGGXXAGUGCA
CUUG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 2 |
1H chemical shifts | 109 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | m62A-h45 | 1 |
Entities:
Entity 1, m62A-h45 24 residues - Formula weight is not available
1 | U | A | A | G | U | G | U | A | C | U | ||||
2 | G | G | DMA | DMA | A | G | U | G | C | A | ||||
3 | C | U | U | G |
Samples:
sample_1: m62A-h45 1 mM
sample_conditions_1: ionic strength: 2 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Uniform NMR System 600 MHz