BMRB Entry 27666
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27666
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Title: Resonance assignment of human LARP4A La module PubMed: 30632004
Deposition date: 2018-10-29 Original release date: 2019-02-06
Authors: Cruz-Gallardo, Isabel; Martino, Luigi; Trotta, Roberta; De Tito, Stefano; Atkinson, Andrew; Conte, Maria
Citation: Cruz-Gallardo, Isabel; Martino, Luigi; Trotta, Roberta; De Tito, Stefano; Atkinson, Andrew; Conte, Maria. "Resonance assignment of human LARP4A La module" Biomol. NMR Assignments 13, 169-172 (2019).
Assembly members:
LARP4A, polymer, 179 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
LARP4A: QSNSAVSTEDLKECLKKQLE
FCFSRENLSKDLYLISQMDS
DQFIPIWTVANMEEIKKLTT
DPDLILEVLRSSPMVQVDEK
GEKVRPSHKRCIVILREIPE
TTPIEEVKGLFKSENCPKVI
SCEFAHNSNWYITFQSDTDA
QQAFKYLREEVKTFQGKPIM
ARIKAINTFFAKNGYRLMD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 543 |
| 15N chemical shifts | 158 |
| 1H chemical shifts | 1035 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LARP4A | 1 |
Entities:
Entity 1, LARP4A 179 residues - Formula weight is not available
| 1 | GLN | SER | ASN | SER | ALA | VAL | SER | THR | GLU | ASP | ||||
| 2 | LEU | LYS | GLU | CYS | LEU | LYS | LYS | GLN | LEU | GLU | ||||
| 3 | PHE | CYS | PHE | SER | ARG | GLU | ASN | LEU | SER | LYS | ||||
| 4 | ASP | LEU | TYR | LEU | ILE | SER | GLN | MET | ASP | SER | ||||
| 5 | ASP | GLN | PHE | ILE | PRO | ILE | TRP | THR | VAL | ALA | ||||
| 6 | ASN | MET | GLU | GLU | ILE | LYS | LYS | LEU | THR | THR | ||||
| 7 | ASP | PRO | ASP | LEU | ILE | LEU | GLU | VAL | LEU | ARG | ||||
| 8 | SER | SER | PRO | MET | VAL | GLN | VAL | ASP | GLU | LYS | ||||
| 9 | GLY | GLU | LYS | VAL | ARG | PRO | SER | HIS | LYS | ARG | ||||
| 10 | CYS | ILE | VAL | ILE | LEU | ARG | GLU | ILE | PRO | GLU | ||||
| 11 | THR | THR | PRO | ILE | GLU | GLU | VAL | LYS | GLY | LEU | ||||
| 12 | PHE | LYS | SER | GLU | ASN | CYS | PRO | LYS | VAL | ILE | ||||
| 13 | SER | CYS | GLU | PHE | ALA | HIS | ASN | SER | ASN | TRP | ||||
| 14 | TYR | ILE | THR | PHE | GLN | SER | ASP | THR | ASP | ALA | ||||
| 15 | GLN | GLN | ALA | PHE | LYS | TYR | LEU | ARG | GLU | GLU | ||||
| 16 | VAL | LYS | THR | PHE | GLN | GLY | LYS | PRO | ILE | MET | ||||
| 17 | ALA | ARG | ILE | LYS | ALA | ILE | ASN | THR | PHE | PHE | ||||
| 18 | ALA | LYS | ASN | GLY | TYR | ARG | LEU | MET | ASP |
Samples:
sample_1: LARP4A, [U-100% 13C; U-100% 15N], 400 uM; LARP4A, [U-100% 15N], 400 uM; TRIS 20 mM; KCL 100 mM; DTT 1 mM; EDTA 0.2 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7.25; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
XEASY, Bartels et al. - chemical shift assignment
CCPN, CCPN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 950 MHz
- Bruker EON 800 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts