BMRB Entry 30032
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30032
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Title: Solution Structure of Miz-1 Zinc Finger 13 PubMed: 26972249
Deposition date: 2016-03-08 Original release date: 2016-03-28
Authors: Tremblay, C.; Bedard, M.; Lavigne, P.
Citation: Tremblay, C.; Bedard, M.; Bonin, MA.; Lavigne, P.. "Solution structure of the 13th C2H2 Zinc Finger of Miz-1" Biochem. Biophys. Res. Commun. 473, 471-475 (2016).
Assembly members:
entity_1, polymer, 29 residues, 3232.629 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: HILYACDSCGDKFLDANSLA
QHVRIHTAQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 65 |
| 15N chemical shifts | 22 |
| 1H chemical shifts | 178 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 29 residues - 3232.629 Da.
| 1 | HIS | ILE | LEU | TYR | ALA | CYS | ASP | SER | CYS | GLY | ||||
| 2 | ASP | LYS | PHE | LEU | ASP | ALA | ASN | SER | LEU | ALA | ||||
| 3 | GLN | HIS | VAL | ARG | ILE | HIS | THR | ALA | GLN |
Entity 2, entity_2 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: ZF 13 0.75 mM; ZnCl2 2 mM; KCl 50 mM; Bis Tris, [U-2H], 10 mM; TECP 2 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.2, Linge, O'Donoghue and Nilges - structure calculation
CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CcpNMR_Analysis, CCPN - chemical shift assignment
DANGLE v1.1, CCPN - data analysis
NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian Unity Inova 600 MHz
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
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or all simulated shifts