BMRB Entry 30155
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30155
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR solution structure of the RRM1 domain of the post-transcriptional regulator HuR
Deposition date: 2016-08-15 Original release date: 2017-08-04
Authors: Lixa, C.; Mujo, A.; Jendiroba, K.; Almeida, F.; Lima, L.; Pinheiro, A.
Citation: Lixa, C.; Mujo, A.; Jendiroba, K.; Almeida, F.; Lima, L.; Pinheiro, A.; Mujo, A.; Lixa, C.; Carneiro, L.; Anobom, C.; Almeida, F.; Pinheiro, A.. "Insights into HuR RRM1 domain self-association and mRNA recognition from solution NMR studies" . ., .-..
Assembly members:
entity_1, polymer, 101 residues, 11220.585 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GHMSNGYEDHMAEDCRGDIG
RTNLIVNYLPQNMTQDELRS
LFSSIGEVESAKLIRDKVAG
HSLGYGFVNYVTAKDAERAI
NTLNGLRLQSKTIKVSYARP
S
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 412 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 666 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 101 residues - 11220.585 Da.
| 1 | GLY | HIS | MET | SER | ASN | GLY | TYR | GLU | ASP | HIS | ||||
| 2 | MET | ALA | GLU | ASP | CYS | ARG | GLY | ASP | ILE | GLY | ||||
| 3 | ARG | THR | ASN | LEU | ILE | VAL | ASN | TYR | LEU | PRO | ||||
| 4 | GLN | ASN | MET | THR | GLN | ASP | GLU | LEU | ARG | SER | ||||
| 5 | LEU | PHE | SER | SER | ILE | GLY | GLU | VAL | GLU | SER | ||||
| 6 | ALA | LYS | LEU | ILE | ARG | ASP | LYS | VAL | ALA | GLY | ||||
| 7 | HIS | SER | LEU | GLY | TYR | GLY | PHE | VAL | ASN | TYR | ||||
| 8 | VAL | THR | ALA | LYS | ASP | ALA | GLU | ARG | ALA | ILE | ||||
| 9 | ASN | THR | LEU | ASN | GLY | LEU | ARG | LEU | GLN | SER | ||||
| 10 | LYS | THR | ILE | LYS | VAL | SER | TYR | ALA | ARG | PRO | ||||
| 11 | SER |
Samples:
sample_1: DTT 10 mM; HuR_RRM1, [U-15N], 1.5 mM; sodium chloride 100 mM; sodium phosphate 30 mM
sample_2: DTT 10 mM; HuR_RRM1, [U-100% 13C; U-100% 15N], 1.5 mM; sodium chloride 100 mM; sodium phosphate 30 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D (H)CC(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HC(C)H-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
CcpNMR, CCPN - data analysis
TOPSPIN v3.1, Bruker Biospin - processing
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AvanceIII 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts