BMRB Entry 30170
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30170
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Title: TIRAP phosphoinositide-binding motif PubMed: 28225045
Deposition date: 2016-09-05 Original release date: 2017-03-02
Authors: Capelluto, D.; Ellena, J.; Armstrong, G.; Zhao, X.; Xiao, S.
Citation: Zhao, X.; Xiong, W.; Xiao, S.; Ellena, J.; Armstrong, G.; Finkielstein, C.; Capelluto, D.. "Membrane targeting of TIRAP is negatively regulated by phosphorylation in its phosphoinositide-binding motif" Sci. Rep. 7, 43043-43043 (2017).
Assembly members:
entity_1, polymer, 21 residues, 2550.200 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Entity Sequences (FASTA):
entity_1: KKPLGKMADWFRQTLLKKPK
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 61 |
| 15N chemical shifts | 19 |
| 1H chemical shifts | 19 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 21 residues - 2550.200 Da.
| 1 | LYS | LYS | PRO | LEU | GLY | LYS | MET | ALA | ASP | TRP | ||||
| 2 | PHE | ARG | GLN | THR | LEU | LEU | LYS | LYS | PRO | LYS | ||||
| 3 | LYS |
Samples:
sample_1: TIRAP PBM, [U-99% 13C; U-99% 15N], 0.4 ± 0.04 mM
sample_conditions_1: ionic strength: 60 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D TOCSYHSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D NOESYHSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CS-Rosetta v3.5, Shen, Vernon, Baker and Bax - refinement, structure calculation
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AvanceIII 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts