BMRB Entry 30311
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30311
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Title: Solution structure of phage displayed derived peptide inhibitor of frizzled 7 receptor PubMed: 29632413
Deposition date: 2017-06-22 Original release date: 2018-04-11
Authors: Fu, Y.
Citation: Nile, Aaron; de Sousa E Melo, Felipe; Mukund, Susmith; Piskol, Robert; Hansen, Simon; Zhou, Lijuan; Zhang, Yingnan; Fu, Yue; Gogol, Emily; Komuves, Laszlo; Modrusan, Zora; Angers, Stephane; Franke, Yvonne; Koth, Christopher; Fairbrother, Wayne; Wang, Weiru; de Sauvage, Frederic; Hannoush, Rami. "A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells" Nat. Chem. Biol. 14, 582-590 (2018).
Assembly members:
Fz7 binding peptide, polymer, 14 residues, 1755.986 Da.
Natural source: Common Name: not available Taxonomy ID: 32644 Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Fz7 binding peptide: LPSDDLEFWCHVMY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 178 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1, chain A | 1 |
| 2 | entity_1, chain B | 1 |
Entities:
Entity 1, entity_1, chain A 14 residues - 1755.986 Da.
| 1 | LEU | PRO | SER | ASP | ASP | LEU | GLU | PHE | TRP | CYS | ||||
| 2 | HIS | VAL | MET | TYR |
Samples:
sample_1: Fz binding peptide 1 mM; acentonitrile 10%; sodium phosphate 10 mM
sample_conditions_1: ionic strength: 10 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Analysis, CCPN - chemical shift assignment, data analysis, peak picking
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
NMR spectrometers:
- Bruker ultrashield plus 600 MHz