BMRB Entry 30504
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30504
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Title: Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa
Deposition date: 2018-07-31 Original release date: 2019-08-02
Authors: Kenward, C.; Langelaan, D.
Citation: Kenward, C.; Langelaan, D.. "Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa" . ., .-..
Assembly members:
entity_1, polymer, 84 residues, 8286.277 Da.
Natural source: Common Name: White-rot fungus Taxonomy ID: 650164 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phanerochaete carnosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli DH1
Entity Sequences (FASTA):
entity_1: GSWGQCSTGSIQCCQNVVPG
DSDLGTLLLDELGIVLEDPT
VLIGDGCDPITVAGSSDACS
ATAVCCSDNNVSGVIAIGCL
PVTL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 326 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 519 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 84 residues - 8286.277 Da.
| 1 | GLY | SER | TRP | GLY | GLN | CYS | SER | THR | GLY | SER | ||||
| 2 | ILE | GLN | CYS | CYS | GLN | ASN | VAL | VAL | PRO | GLY | ||||
| 3 | ASP | SER | ASP | LEU | GLY | THR | LEU | LEU | LEU | ASP | ||||
| 4 | GLU | LEU | GLY | ILE | VAL | LEU | GLU | ASP | PRO | THR | ||||
| 5 | VAL | LEU | ILE | GLY | ASP | GLY | CYS | ASP | PRO | ILE | ||||
| 6 | THR | VAL | ALA | GLY | SER | SER | ASP | ALA | CYS | SER | ||||
| 7 | ALA | THR | ALA | VAL | CYS | CYS | SER | ASP | ASN | ASN | ||||
| 8 | VAL | SER | GLY | VAL | ILE | ALA | ILE | GLY | CYS | LEU | ||||
| 9 | PRO | VAL | THR | LEU |
Samples:
sample_1: PC1, [U-13C; U-15N], 800 uM; MES 20 mM; sodium chloride 50 mM; sodium azide 1 mM
sample_2: PC1, [U-15N], 110 uM; MES 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 303.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
ARIA v2.3.1, Linge, O'Donoghue and Nilges - refinement, structure calculation
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Analysis, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AvanceIII 800 MHz
- Bruker AvanceIII 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts