BMRB Entry 30507
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30507
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Title: Cytokine-like Peptide Stress-response Peptide-1 from Manduca Sexta
Deposition date: 2018-08-13 Original release date: 2019-08-16
Authors: Schrag, L.
Citation: Schrag, L.; Cao, X.; Al Souhail, Q.; Kanost, M.; Jiang, H.; Prakash, O.. "NMR Solution Structure and Expression Profile of Stress Response Peptide-1: A Cytokine from Manduca sexta" To be published ., .-..
Assembly members:
entity_1, polymer, 25 residues, 2818.129 Da.
Natural source: Common Name: tobacco hornworm Taxonomy ID: 7130 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Manduca sexta
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: FGVRVGTCPSGYVRRGTFCF
PDDDY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 149 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 25 residues - 2818.129 Da.
| 1 | PHE | GLY | VAL | ARG | VAL | GLY | THR | CYS | PRO | SER | ||||
| 2 | GLY | TYR | VAL | ARG | ARG | GLY | THR | PHE | CYS | PHE | ||||
| 3 | PRO | ASP | ASP | ASP | TYR |
Samples:
sample_1: Stress-response Peptide-1 1.8 ± 0.1 mM
sample_conditions_1: pH: 2.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
Software:
VNMR v3.2a, Varian - collection, processing
CcpNMR v2.4, CCPN - peak picking
Analysis v2.4, CCPN - chemical shift assignment
CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
ProcheckNMR, Laskowski and MacArthur - refinement
NMR spectrometers:
- Varian UNITY 500 MHz