BMRB Entry 30551
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30551
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Title: Solution Structure of the Thioredoxin-like Domain of Arabidopsis NCP PubMed: 31201314
Deposition date: 2018-12-17 Original release date: 2019-06-17
Authors: Liu, J.; Chen, M.; Zhou, P.
Citation: Yang, Emily; Yoo, Chan Yul; Liu, Jiangxin; Wang, He; Cao, Jun; Li, Fay-Wei; Pryer, Kathleen; Sun, Tai-Ping; Weigel, Detlef; Zhou, Pei; Chen, Meng. "NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches" Nat. Commun. 10, 2630-2630 (2019).
Assembly members:
entity_1, polymer, 146 residues, 16605.234 Da.
Natural source: Common Name: Mouse-ear cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: SHMDNYIRPIKDLTTAEWEE
AVFKDISPLMVLVHNRYKRP
KENEKFREELEKAIQVIWNC
GLPSPRCVAVDAVVETDLVS
ALKVSVFPEIIFTKAGKILY
REKGIRTADELSKIMAFFYY
GAAKPPCLNGVVNSQEQIPL
VDVSVN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 503 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 1058 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 146 residues - 16605.234 Da.
| 1 | SER | HIS | MET | ASP | ASN | TYR | ILE | ARG | PRO | ILE | ||||
| 2 | LYS | ASP | LEU | THR | THR | ALA | GLU | TRP | GLU | GLU | ||||
| 3 | ALA | VAL | PHE | LYS | ASP | ILE | SER | PRO | LEU | MET | ||||
| 4 | VAL | LEU | VAL | HIS | ASN | ARG | TYR | LYS | ARG | PRO | ||||
| 5 | LYS | GLU | ASN | GLU | LYS | PHE | ARG | GLU | GLU | LEU | ||||
| 6 | GLU | LYS | ALA | ILE | GLN | VAL | ILE | TRP | ASN | CYS | ||||
| 7 | GLY | LEU | PRO | SER | PRO | ARG | CYS | VAL | ALA | VAL | ||||
| 8 | ASP | ALA | VAL | VAL | GLU | THR | ASP | LEU | VAL | SER | ||||
| 9 | ALA | LEU | LYS | VAL | SER | VAL | PHE | PRO | GLU | ILE | ||||
| 10 | ILE | PHE | THR | LYS | ALA | GLY | LYS | ILE | LEU | TYR | ||||
| 11 | ARG | GLU | LYS | GLY | ILE | ARG | THR | ALA | ASP | GLU | ||||
| 12 | LEU | SER | LYS | ILE | MET | ALA | PHE | PHE | TYR | TYR | ||||
| 13 | GLY | ALA | ALA | LYS | PRO | PRO | CYS | LEU | ASN | GLY | ||||
| 14 | VAL | VAL | ASN | SER | GLN | GLU | GLN | ILE | PRO | LEU | ||||
| 15 | VAL | ASP | VAL | SER | VAL | ASN |
Samples:
sample_1: NCP Thioredoxin-like Domain, [U-99% 13C; U-99% 15N], 1.2 mM; HEPES 25 mM; KCl 50 mM; DTT 10 mM
sample_2: NCP Thioredoxin-like Domain, [U-99% 13C; U-99% 15N], 1.2 mM; HEPES 25 mM; KCl 50 mM; DTT 10 mM
sample_3: NCP Thioredoxin-like Domain, [U-99% 15N], 1.2 mM; HEPES 25 mM; KCl 50 mM; DTT 10 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Triple-resonance | sample_1 | isotropic | sample_conditions_1 |
| 4D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 4D HCCONH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_3 | isotropic | sample_conditions_1 |
| 4D 13C-HMQC-NOESY-15N-HSQC | sample_1 | isotropic | sample_conditions_1 |
| 4D 13C-HMQC-NOESY-13C-HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts