BMRB Entry 30566
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30566
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Title: hGRNA4-28_3s
Deposition date: 2019-02-01 Original release date: 2020-01-30
Authors: Dastpeyman, M.; Bansal, P.; Wilson, D.; Loukas, A.; Smout, M.; Daly, N.
Citation: Dastpeyman, M.; Bansal, P.; Wilson, D.; Sotillo, J.; Brindley, P.; Loukas, A.; Smout, M.; Daly, N.. "hGRN4-28_3s" to be published ., .-..
Assembly members:
entity_1, polymer, 24 residues, 2588.959 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: CDMEVSCPDGYTCCRLQSGA
WGCC
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 24 residues - 2588.959 Da.
| 1 | CYS | ASP | MET | GLU | VAL | SER | CYS | PRO | ASP | GLY | ||||
| 2 | TYR | THR | CYS | CYS | ARG | LEU | GLN | SER | GLY | ALA | ||||
| 3 | TRP | GLY | CYS | CYS |
Samples:
sample_1: hGRNA4-28_3s 0.5 mM
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5PL6, Bruker Biospin - collection
CYANA v3.97, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
CcpNMR, CCPN - chemical shift assignment, data analysis
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz