BMRB Entry 30567
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30567
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Title: NMR Structure of the DNA binding domain of EhMybS3
Deposition date: 2019-02-05 Original release date: 2020-02-07
Authors: Titaux-Delgado, G.; del Rio-Portilla, F.
Citation: Cardenas-Hernandez, H.; Titaux-Delgado, G.; Castaneda-Ortiz, E.; Torres-Larios, A.; Brieba, L.; de Rio-portilla, F.; Azuara-Liceaga, E.. "Biosynthesis and structure analysis of the DNA binding domain of Plant-like Myb protein in Entamoeba histolytica" . ., .-..
Assembly members:
entity_1, polymer, 60 residues, 6986.167 Da.
Natural source: Common Name: Entamoeba histolytica Taxonomy ID: 5759 Superkingdom: Eukaryota Kingdom: not available Genus/species: Entamoeba histolytica
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GPLGSKKREVWTDAEHAKFV
EGLALFHKDWKKIKEYIGTK
TVVQIRSHAQKYFLKLNKTA
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 355 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 60 residues - 6986.167 Da.
| 1 | GLY | PRO | LEU | GLY | SER | LYS | LYS | ARG | GLU | VAL | |
| 2 | TRP | THR | ASP | ALA | GLU | HIS | ALA | LYS | PHE | VAL | |
| 3 | GLU | GLY | LEU | ALA | LEU | PHE | HIS | LYS | ASP | TRP | |
| 4 | LYS | LYS | ILE | LYS | GLU | TYR | ILE | GLY | THR | LYS | |
| 5 | THR | VAL | VAL | GLN | ILE | ARG | SER | HIS | ALA | GLN | |
| 6 | LYS | TYR | PHE | LEU | LYS | LEU | ASN | LYS | THR | ALA |
Samples:
sample_1: EhMybS3 1.5 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA v1.8, Keller and Wuthrich - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker AMX 700 MHz