BMRB Entry 30592
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30592
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR structure of Orf47 from Lactococcus virus P2
Deposition date: 2019-03-21 Original release date: 2020-03-27
Authors: Gagne, S.
Citation: Gagne, S.. "Structure determination of ORF47 from Lactococcus virus P2" . ., .-..
Assembly members:
entity_1, polymer, 43 residues, 5137.874 Da.
Natural source: Common Name: Lactococcus phage p2 Taxonomy ID: 254252 Superkingdom: Viruses Kingdom: not available Genus/species: Skunavirus Lactococcus phage p2
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: MNKEHILAQKEVLTPIEYEH
YVKHLFDIGEITKELYIELS
SDL
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 114 |
| 15N chemical shifts | 35 |
| 1H chemical shifts | 276 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 43 residues - 5137.874 Da.
| 1 | MET | ASN | LYS | GLU | HIS | ILE | LEU | ALA | GLN | LYS | ||||
| 2 | GLU | VAL | LEU | THR | PRO | ILE | GLU | TYR | GLU | HIS | ||||
| 3 | TYR | VAL | LYS | HIS | LEU | PHE | ASP | ILE | GLY | GLU | ||||
| 4 | ILE | THR | LYS | GLU | LEU | TYR | ILE | GLU | LEU | SER | ||||
| 5 | SER | ASP | LEU |
Samples:
sample_1: entity_1 mM; potassium phosphate 20 mM; potassium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts