BMRB Entry 30625
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30625
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Antimicrobial lasso peptide ubonodin PubMed: 31765515
Deposition date: 2019-07-05 Original release date: 2019-12-02
Authors: Link, A.; Cheung-Lee, W.
Citation: Cheung-Lee, W.; Parry, M.; Zong, C.; Cartagena, A.; Darst, S.; Connell, N.; Russo, R.; Link, A.. "Discovery of ubonodin, an antimicrobial lasso peptide active against members of the Burkholderia cepacia complex" Chembiochem. ., .-. (2019).
Assembly members:
entity_1, polymer, 28 residues, 3220.489 Da.
Natural source: Common Name: Burkholderia ubonensis Taxonomy ID: 101571 Superkingdom: Bacteria Kingdom: not available Genus/species: Burkholderia ubonensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21
Entity Sequences (FASTA):
entity_1: GGDGSIAEYFNRPMHIHDWQ
IMDSGYYG
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 172 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 28 residues - 3220.489 Da.
| 1 | GLY | GLY | ASP | GLY | SER | ILE | ALA | GLU | TYR | PHE | ||||
| 2 | ASN | ARG | PRO | MET | HIS | ILE | HIS | ASP | TRP | GLN | ||||
| 3 | ILE | MET | ASP | SER | GLY | TYR | TYR | GLY |
Samples:
sample_1: Ubonodin 9 mg/mL
sample_2: Ubonodin 4.6 mg/mL
sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 295.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
MestreLab (Mnova / MestReNova / MestReC) v11.0, Mestrelab Research - processing
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure calculation
GROMACS, HERMAN BERENDSEN'S GROUP - refinement
NMR spectrometers:
- Bruker AVANCE III 800 MHz