BMRB Entry 30631
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR30631
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Title: Solution structure of OlvA(BC)
Deposition date: 2019-07-09 Original release date: 2019-10-14
Authors: Acedo, J.; van der Donk, W.
Citation: Acedo, J.; van der Donk, W.. "O-Methyltransferase-mediated Incorporation of a b-Amino Acid in Lanthipeptides" . ., .-..
Assembly members:
entity_1, polymer, 19 residues, 1616.841 Da.
Natural source: Common Name: Streptomyces olivaceus Taxonomy ID: 47716 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces olivaceus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: ACGXGDGCAKXCAASCAAS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 84 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 19 residues - 1616.841 Da.
| 1 | ALA | CYS | GLY | DBB | GLY | ASP | GLY | CYS | ALA | LYS | ||||
| 2 | DBB | CYS | ALA | ALA | SER | CYS | ALA | ALA | SER |
Samples:
sample_1: OlvA(BC) 2.5 mM; sodium phosphate 30 mM; DSS 0.001%
sample_2: OlvA(BC) 2.5 mM; sodium phosphate 30 mM; DSS 0.001%
sample_conditions_1: ionic strength: 30 mM; pH: 5.8; pressure: 760 mmHg; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 750 MHz