BMRB Entry 30633
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30633
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Title: N-terminal domain of dynein intermediate chain from Chaetomium thermophilum
Deposition date: 2019-07-10 Original release date: 2020-08-12
Authors: Loening, N.; Barbar, E.
Citation: Loening, Nikolaus; Saravanan, Sanjana; Jespersen, Nathan; Jara, Kayla; Barbar, Elisar. "Interplay of disorder and sequence specificity in the formation of stable dynein/dynactin complexes" Biophy. J. ., .-..
Assembly members:
entity_1, polymer, 92 residues, 10013.260 Da.
Natural source: Common Name: Chaetomium thermophilum Taxonomy ID: 759272 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium thermophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: GAHMMQARREELLAKKARLA
EIKRQRELRAQQAAGRSITP
SELVSPTPSRANSRREIESL
IDSILSSSAGANSPRRGSRP
NSVISTGELSTD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 390 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 625 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 92 residues - 10013.260 Da.
| 1 | GLY | ALA | HIS | MET | MET | GLN | ALA | ARG | ARG | GLU | ||||
| 2 | GLU | LEU | LEU | ALA | LYS | LYS | ALA | ARG | LEU | ALA | ||||
| 3 | GLU | ILE | LYS | ARG | GLN | ARG | GLU | LEU | ARG | ALA | ||||
| 4 | GLN | GLN | ALA | ALA | GLY | ARG | SER | ILE | THR | PRO | ||||
| 5 | SER | GLU | LEU | VAL | SER | PRO | THR | PRO | SER | ARG | ||||
| 6 | ALA | ASN | SER | ARG | ARG | GLU | ILE | GLU | SER | LEU | ||||
| 7 | ILE | ASP | SER | ILE | LEU | SER | SER | SER | ALA | GLY | ||||
| 8 | ALA | ASN | SER | PRO | ARG | ARG | GLY | SER | ARG | PRO | ||||
| 9 | ASN | SER | VAL | ILE | SER | THR | GLY | GLU | LEU | SER | ||||
| 10 | THR | ASP |
Samples:
sample_1: CT-IC 1-88, [U-99% 13C; U-99% 15N], 200 uM; NaCl 50 mM; phosphate 50 mM; sodium azide 1 mM; DSS 0.2 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts