BMRB Entry 30651
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30651
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Title: Lasso peptide pandonodin PubMed: 31742991
Deposition date: 2019-08-06 Original release date: 2019-12-02
Authors: Link, A.; Cheung-Lee, W.
Citation: Cheung-Lee, W.; Cao, L.; Link, A.. "Pandonodin: a proteobacterial lasso peptide with an exceptionally long C-terminal tail." Acs Chem. Biol. ., .-. (2019).
Assembly members:
entity_1, polymer, 33 residues, 3330.956 Da.
Natural source: Common Name: Pandoraea norimbergensis Taxonomy ID: 93219 Superkingdom: Bacteria Kingdom: not available Genus/species: Pandoraea norimbergensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21
Entity Sequences (FASTA):
entity_1: GVLGNDAEGITLLPLCFKPI
CIPTLPPLTGGHA
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 235 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 33 residues - 3330.956 Da.
| 1 | GLY | VAL | LEU | GLY | ASN | ASP | ALA | GLU | GLY | ILE | ||||
| 2 | THR | LEU | LEU | PRO | LEU | CYS | PHE | LYS | PRO | ILE | ||||
| 3 | CYS | ILE | PRO | THR | LEU | PRO | PRO | LEU | THR | GLY | ||||
| 4 | GLY | HIS | ALA |
Samples:
sample_1: pandonodin 6 mg/mL
sample_conditions_1: ionic strength: 0 Not defined; pH: 6.9; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D gCOSY | sample_1 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
MestreLab (Mnova / MestReNova / MestReC) v11.0, Mestrelab Research - chemical shift assignment, peak picking, processing
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure calculation
NMR spectrometers:
- Bruker AVANCE III 800 MHz