BMRB Entry 3322
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR3322
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Title: Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi PubMed: 11524680
Deposition date: 2001-09-10 Original release date: 1995-07-31
Authors: Raghothama, Srinivasrao; Eberhardt, Ruth; Simpson, Peter; Wigelsworth, Darran; White, Peter; Hazlewood, Geoffrey; Nagy, Tibor; Gilbert, Harry; Williamson, Michael
Citation: Raghothama, Srinivasrao; Eberhardt, Ruth; Simpson, Peter; Wigelsworth, Darran; White, Peter; Hazlewood, Geoffrey; Nagy, Tibor; Gilbert, Harry; Williamson, Michael. "Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi" Nat. Struct. Biol. 8, 775-778 (2001).
Assembly members:
dockerin, polymer, 52 residues, Formula weight is not available
Natural source: Common Name: Piromyces equi Taxonomy ID: 99929 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Piromyces equi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
dockerin: GSCWAQSQGYNCCNNPSSTK
VEYTDASGQWGVQNGQWCGI
DYSYGQNQGNES
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 301 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dockerin | 1 |
Entities:
Entity 1, dockerin 52 residues - Formula weight is not available
| 1 | GLY | SER | CYS | TRP | ALA | GLN | SER | GLN | GLY | TYR | ||||
| 2 | ASN | CYS | CYS | ASN | ASN | PRO | SER | SER | THR | LYS | ||||
| 3 | VAL | GLU | TYR | THR | ASP | ALA | SER | GLY | GLN | TRP | ||||
| 4 | GLY | VAL | GLN | ASN | GLY | GLN | TRP | CYS | GLY | ILE | ||||
| 5 | ASP | TYR | SER | TYR | GLY | GLN | ASN | GLN | GLY | ASN | ||||
| 6 | GLU | SER |
Samples:
Sample_1: dockerin, [U-15N], 1 mM
condition_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D-HSQC/NOESY | Sample_1 | not available | condition_1 |
| HNHA | Sample_1 | not available | condition_1 |
| HNHB | Sample_1 | not available | condition_1 |
| 2D-HSQC | Sample_1 | not available | condition_1 |
| 15N decoupled NOESY | Sample_1 | not available | condition_1 |
| TOCSY | Sample_1 | not available | condition_1 |
| DQF-COSY | Sample_1 | not available | condition_1 |
| E.COSY | Sample_1 | not available | condition_1 |
Software:
FELIX -
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
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