BMRB Entry 34013

Name: Solution structure of BOLA1 from Homo sapiens
Published: 2016-08-26

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PDB ID: 5lci
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34013
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of BOLA1 from Homo sapiens PubMed: 27532772

Deposition date: 2016-06-21 Original release date: 2016-08-26

Authors: Nasta, V.; Ciofi Baffoni, S.; Banci, L.

Citation: Uzarska, M.; Nasta, V.; Weiler, B.; Spantgar, F.; Ciofi-Baffoni, S.; Saviello, M.; Gonnelli, L.; M hlenhoff, U.; Banci, L.; Lill, R.. "Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins" Elife 5, e16673-e16673 (2016).

Assembly members:
BolA-like protein 1, polymer, 123 residues, 12875.399 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
BolA-like protein 1: GIDPFTQGSAGSGAIGPVEA AIRTKLEEALSPEVLELRNE SGGHAVPPGSETHFRVAVVS SRFEGLSPLQRHRLVHAALA EELGGPVHALAIQARTPAQW RENSQLDTSPPCLGGNKKTL GTP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	465
15N chemical shifts	126
1H chemical shifts	778

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 123 residues - 12875.399 Da.

1

GLY

ILE

ASP

PRO

PHE

THR

GLN

GLY

SER

ALA

2

GLY

SER

GLY

ALA

ILE

GLY

PRO

VAL

GLU

ALA

3

ALA

ILE

ARG

THR

LYS

LEU

GLU

ALA

LEU

4

SER

PRO

GLU

VAL

LEU

GLU

LEU

ARG

ASN

GLU

5

SER

GLY

HIS

ALA

VAL

PRO

GLY

SER

6

GLU

THR

HIS

PHE

ARG

VAL

ALA

VAL

SER

7

SER

ARG

PHE

GLU

GLY

LEU

SER

PRO

LEU

GLN

8

ARG

HIS

ARG

LEU

VAL

HIS

ALA

LEU

ALA

9

GLU

LEU

GLY

PRO

VAL

HIS

ALA

LEU

10

ALA

ILE

GLN

ALA

ARG

THR

PRO

ALA

GLN

TRP

11

ARG

GLU

ASN

SER

GLN

LEU

ASP

THR

SER

PRO

12

PRO

CYS

LEU

GLY

ASN

LYS

THR

LEU

13

GLY

THR

PRO

Samples:

sample_1: DTT 5 mM; mitochondrial BOLA1 protein, [U-100% 15N], 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_2: DTT 5 mM; mitochondrial BOLA1 protein, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	anisotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	anisotropic	sample_conditions_2
3D HNCO	sample_2	anisotropic	sample_conditions_2
3D HNCA	sample_2	anisotropic	sample_conditions_2
3D HN(CO)CA	sample_2	anisotropic	sample_conditions_2
3D HBHA(CBCACO)NH	sample_2	anisotropic	sample_conditions_2
3D HN(CA)CO	sample_2	anisotropic	sample_conditions_2
3D CBCA(CO)NH	sample_2	anisotropic	sample_conditions_2
3D HNCACB	sample_2	anisotropic	sample_conditions_2
3D HNCACB	sample_2	anisotropic	sample_conditions_2
3D 1H-13C NOESY	sample_2	anisotropic	sample_conditions_2
3D HCCH-TOCSY	sample_2	anisotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_1	anisotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	anisotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	anisotropic	sample_conditions_1

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CARA, Keller and Wuthrich - peak picking

CARA v2, Keller and Wuthrich - chemical shift assignment

TALOS+, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection, processing

UNIO, Serrano, Pedrini, Mohanty, Geralt, Herrmann and Wuthrich, - peak picking, structure calculation

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 900 MHz
Bruker Avance 950 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts