BMRB Entry 34080
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34080
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Title: hnRNP A1 RRM2 in complex with 5'-UCAGUU-3' RNA PubMed: 28650318
Deposition date: 2016-12-16 Original release date: 2017-06-30
Authors: Barraud, P.; Allain, F.
Citation: Beusch, Irene; Barraud, Pierre; Moursy, Ahmed; Clery, Antoine; Allain, Frederic Hai-Trieu. "Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7" Elife 6, e25736-e25736 (2017).
Assembly members:
Heterogeneous nuclear ribonucleoprotein A1, polymer, 102 residues, 11630.084 Da.
RNA UCAGUU, polymer, 6 residues, 1853.133 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Heterogeneous nuclear ribonucleoprotein A1: SQRPGAHLTVKKIFVGGIKE
DTEEHHLRDYFEQYGKIEVI
EIMTDRGSGKKRGFAFVTFD
DHDSVDKIVIQKYHTVNGHN
CEVRKALSKQEMASASSSQR
GR
RNA UCAGUU: UCAGUU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 654 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 102 residues - 11630.084 Da.
| 1 | SER | GLN | ARG | PRO | GLY | ALA | HIS | LEU | THR | VAL | ||||
| 2 | LYS | LYS | ILE | PHE | VAL | GLY | GLY | ILE | LYS | GLU | ||||
| 3 | ASP | THR | GLU | GLU | HIS | HIS | LEU | ARG | ASP | TYR | ||||
| 4 | PHE | GLU | GLN | TYR | GLY | LYS | ILE | GLU | VAL | ILE | ||||
| 5 | GLU | ILE | MET | THR | ASP | ARG | GLY | SER | GLY | LYS | ||||
| 6 | LYS | ARG | GLY | PHE | ALA | PHE | VAL | THR | PHE | ASP | ||||
| 7 | ASP | HIS | ASP | SER | VAL | ASP | LYS | ILE | VAL | ILE | ||||
| 8 | GLN | LYS | TYR | HIS | THR | VAL | ASN | GLY | HIS | ASN | ||||
| 9 | CYS | GLU | VAL | ARG | LYS | ALA | LEU | SER | LYS | GLN | ||||
| 10 | GLU | MET | ALA | SER | ALA | SER | SER | SER | GLN | ARG | ||||
| 11 | GLY | ARG |
Entity 2, entity_2 6 residues - 1853.133 Da.
| 1 | U | C | A | G | U | U |
Samples:
sample_1: RNA UCAGUU 1.0 g/L; hnRNP A1 RRM2, [U-99% 15N], 1.0 mM; H2O 90%; D2O 10%
sample_2: RNA UCAGUU 1.0 mM; hnRNP A1 RRM2, [U-99% 13C; U-99% 15N], 1.0 mM; H2O 90%; D2O 10%
sample_3: RNA UCAGUU 1.0 mM; hnRNP A1 RRM2, [U-99% 15N], 1.0 mM; D2O 100%
sample_4: RNA UCAGUU 1.0 mM; hnRNP A1 RRM2, [U-99% 13C; U-99% 15N], 1.0 mM; D2O 100%
sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_4 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HCc(CO)NH TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D hCC(CO)NH TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D F1eF3f 13C-filtered/edited NOESY | sample_4 | isotropic | sample_conditions_1 |
| 2D F1fF2f 13C-filtered NOESY | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_3 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
ProcheckNMR, Laskowski and MacArthur - data analysis
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker AvanceIII 500 MHz
- Bruker AvanceIII 600 MHz
- Bruker AvanceIII 750 MHz
- Bruker AvanceIII 700 MHz
- Bruker Avance 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts