BMRB Entry 34129
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34129
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Title: Structure of Tau(254-268) bound to F-actin
Deposition date: 2017-05-04 Original release date: 2018-03-09
Authors: Fontela, Y.; Kadavath, H.; Zweckstetter, M.
Citation: Fontela, Y.; Kadavath, H.; Biernat, J.; Riedel, D.; Mandelkow, E.; Zweckstetter, M.. "Structure of Tau(254-268) bound to F-actin" . ., .-..
Assembly members:
entity_1, polymer, 15 residues, 1687.958 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KNVKSKIGSTENLKH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 14 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 15 residues - 1687.958 Da.
| 1 | LYS | ASN | VAL | LYS | SER | LYS | ILE | GLY | SER | THR | ||||
| 2 | GLU | ASN | LEU | LYS | HIS |
Samples:
sample_1: Tau(254-268) 800 uM; F-actin 27 uM; sodium phosphate 50 mM
sample_2: Tau(254-268) 800 uM; sodium phosphate 50 mM
sample_conditions_1: pH: 6.8; pressure: 1 bar; temperature: 278 K
sample_conditions_2: pH: 6.8; pressure: 1 bar; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
SPARKY, Goddard - chemical shift assignment, peak picking
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker AvanceIII 900 MHz
- Bruker AvanceIII 800 MHz